Recombinant Human Interleukin-2 Receptor Beta （IL-2 Rβ）
Purified No Carrier Protein
（IL2RB, CD122, P70-75）
Prod. No.: I-553
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Interleukin-2 receptor beta （IL-2 Rβ）, also known as IL2RB and CD122, is a type I membrane protein and member of the cytokine receptor superfamily. It forms the high affinity IL-2 receptor along with IL-2 Rα （CD25） and IL-2 Rγ （CD132）. The IL-2 receptor, which is involved in T cell-mediated immune responses, is present in three forms with respect to ability to bind IL-2 （1）. The low affinity form is a monomer of the α subunit and is not involved in signal transduction. The intermediate affinity form consists of an α/β subunit heterodimer, while the high affinity form consists of an α/β/γ heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from IL-2. In spite of the lack of any apparent catalytic motifs, IL2 Rβ is the subunit critical for receptor-mediated signaling via physically or functionally coupling to other signaling molecules, such as the Jak-STAT and Src-family protein tyrosine kinase （2）. IL-2 Rβ is expressed on both circulating T lymphocytes and large granular lymphocytes. It mediates the initial phase of induction of lymphokine activated killer （LAK）, natural killer （NK）, and proliferative activities （3）. Soluble IL2 Rβ binds with IL-15 with sufficient affinity to neutralize its biological activities.

Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-2 Rβ is Mr 24.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 29-31 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
avng tsqftcfyns raniscvwsq dgalqdtscq vhawpdrrrw nqtcellpvs qaswacnlil gapdsqkltt vdivtlrvlc regvrwrvma iqdfkpfenl rlmapislqv vhvethrcni sweisqashy ferhlefear tlspghtwee aplltlkqkq ewicletltp dtqyefqvrv kplqgefttw spwsqplafr tkpaalgkd

Country of Origin
USA
References
1. Burchill, M.A. et al. （2007） J. Immunol. 178:280
2. Friedmann, M.C. et al. （1996） Proc. Natl. Acad. Sci. （USA） 93:2077
3. Leonard, W. J. et al. （1987） Science 238:75

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