蛋白名稱:TNFRSF1A人重組蛋白，10872-H27H-B
蛋白構(gòu)建:A DNA sequence encoding the Human TNFRSF1A (NP_001056.1) (Met1-Thr211) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表達(dá)宿主:HEK293 Cells
蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Immobilized TNF-alpha Protein, Human, Recombinant, HPLC-verified (Cat:10602-HNAE ) at 2 μg/mL (100 μl/well) can bind Recombinant Human TNFR1/CD120a Protein (AVI & His Tag), Biotinylated (Cat: 10872-H27H-B), the EC50 is 2-6 ng/mL.
蛋白內(nèi)毒素:< 1.0 EU per μg protein as determined by the LAL method.
預(yù)測(cè)N端:Ile 22
蛋白分子量:The recombinant Human TNFRSF1A consists of 216 amino acids and predicts a molecular mass of 24.5 kDa. It migrates as an approximately 40.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP號(hào):NP_001056.1
蛋白氨基酸序列:Met1-Thr211
蛋白標(biāo)簽:C-AVI & His

義翹神州是重組蛋白研發(fā)領(lǐng)-導(dǎo)者：

一、技術(shù)平臺(tái)

①五個(gè)重組表達(dá)系統(tǒng)

②從多個(gè)物種中制備的蛋白質(zhì)，以支持動(dòng)物模型和藥物開(kāi)發(fā)

③80+ 自動(dòng)生物反應(yīng)器

④2L-2000L生物反應(yīng)器，確保高通量生產(chǎn)

⑤GMP級(jí)別的生產(chǎn)實(shí)驗(yàn)室和設(shè)施

⑥毫克到克級(jí)的生產(chǎn)規(guī)模

二、嚴(yán)格的質(zhì)量控制體系

①400多種生物活性檢測(cè)和分析方法

②分析設(shè)備，確保生物活性

③N端氨基酸序列分析能力

④高穩(wěn)定性，確保實(shí)驗(yàn)的可重復(fù)性

三、高品質(zhì)重組蛋白產(chǎn)品

①85%以上由真核細(xì)胞表達(dá)，接近結(jié)構(gòu)

②經(jīng)驗(yàn)證的生物活性

③低內(nèi)毒素水平：<0.1EU/μg，采用LAL法確定

④高純度：>95%，采用十二烷基*聚丙烯酰胺凝膠電泳（SDS-PAGE）法或高效液相色譜（HPLC）法確定

⑤不含載體：溶于PBS緩沖液，不含牛血清白蛋白（BSA）

……

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