蛋白名稱:TGFB1人重組蛋白，10804-H08H

蛋白構(gòu)建:A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag.

表達宿主:HEK293 Cells

蛋白純度:> 95 % as determined by SDS-PAGE

蛋白活性:

1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His (Cat: 10804-H08H) at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc (Cat: 10358-H03H), the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL. 

2.Loaded Recombinant Human TGF-beta RII/TGFBR2 Protein, hFc Tag (Cat. No. 10358-H02H ) on ProA Biosensor, can bind Recombinant Human TGF beta 1 Protein (Latent), His Tag (Cat. No. 10804-H08H) with an affinity constant of 47.1 nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).

蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method

預測N端:Leu 30 & Ala 279

蛋白分子量:The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.

蛋白NP號:NP_000651.3

蛋白氨基酸序列:Met1-Ser390

蛋白標簽:C-His

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義翹神州研發(fā)團隊擁有多年的重組蛋白研發(fā)經(jīng)驗。多年來，我們的團隊已開發(fā)出超過9300余種重組蛋白，涵蓋免疫檢查點、抗體藥物靶點、CAR-T細胞治療靶點、Fc受體、流感病毒蛋白以及細胞因子等熱門研究領(lǐng)域。義翹神州重組蛋白具有高純度、高生物活性和低內(nèi)毒素水平等特性，可為生物藥靶點發(fā)現(xiàn)、蛋白質(zhì)結(jié)構(gòu)和功能分析、細胞療法和重組酶酶學性質(zhì)等科學研究提供支持。

義翹神州利用蛋白表達平臺，提供重組蛋白生產(chǎn)"一站式"技術(shù)服務。我們的服務包括蛋白基因合成、蛋白質(zhì)密碼子優(yōu)化、蛋白質(zhì)表達載體設計、大規(guī)模細胞培養(yǎng)和發(fā)酵、蛋白純化和蛋白質(zhì)量控制檢測。我們在蛋白表達和大規(guī)模生產(chǎn)方面有著顯著的成本節(jié)約和速度優(yōu)勢，同時在設計各種類型的重組蛋白純化項目（帶或不帶純化標簽）方面也擁有著豐富的經(jīng)驗和專業(yè)技術(shù)。 義翹神州擁有經(jīng)驗豐富的技術(shù)團隊，每年可研發(fā)生產(chǎn)1,000+種重組蛋白開發(fā)和大規(guī)模生產(chǎn)項目。我們有能力進行快速的蛋白質(zhì)表達和純化工藝開發(fā)，根據(jù)需求進行工藝放大研究，并且可滿足高通量和大規(guī)模批量生產(chǎn)蛋白質(zhì)的要求。 我們致力于成為重組蛋白和單克隆抗體大規(guī)模生產(chǎn)的實驗室，為藥物研發(fā)企業(yè)和生命科學機構(gòu)提供高水平的技術(shù)服務。

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