| 參考價(jià) | ￥550.00 |
| 規(guī)格 | 50 mg | 550元 | 10000 支可售 |
產(chǎn)品簡(jiǎn)介
| CAS號(hào) | 5142-23-4 | 產(chǎn)地 | 進(jìn)口 |
| 級(jí)別 | 其他 |
3-Methyladenine (3-甲基腺嘌呤）
注：本產(chǎn)品僅用于科研，不可用于臨床
生物活性
3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K
IC50 & Target[1]
PtdIns3Kγ 60 μM (IC50, Cell Assay) | Vps34 25 μM (IC50, Cell Assay) | Autophagy
| Mitophagy
| Human Endogenous Metabolite
|
體外研究
(In Vitro)
3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:
在體外細(xì)胞培養(yǎng)實(shí)驗(yàn)中，3-MA 常用的濃度為 0.5-10 mM，所以優(yōu)先推薦直接稱取本次實(shí)驗(yàn)所需粉末量，用培養(yǎng)基全都溶解，0.22 μm 過濾器過濾除菌之后使用。建議溶液現(xiàn)配現(xiàn)用，盡快用完。
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[2]
| Cell Line: | HeLa cells |
| Concentration: | 0 mM, 2.5 mM or 5 mM, 10 mM |
| Incubation Time: | 0 hour, 24 hours and 48 hours |
| Result: | Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation. |
體內(nèi)研究
(In Vivo)
3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290?g)[3] |
| Dosage: | 1.5?mg/100?g (1000 μM) |
| Administration: | Intraperitoneal injection |
| Result: | Alleviated Sodium Taurocholate-Induced SAP. |
分子量
149.15
性狀
Solid
Formula
C6H7N5
CAS 號(hào)
5142-23-4
中文名稱
3-甲基腺嘌呤
運(yùn)輸條件
Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
關(guān)聯(lián)品牌
MCE(MedChemExpress)是科研化學(xué)品和生物活性化合物供應(yīng)商。MCE的產(chǎn)品范圍覆蓋各種抑制劑、化合物庫(kù)、重組蛋白等各類活性分子。我們專注于生物活性化合物，擁有多年的發(fā)展歷程和豐富的行業(yè)經(jīng)驗(yàn)，專業(yè)、高效的企業(yè)理念鑄造了MCE在行業(yè)的地位。我們的研發(fā)團(tuán)隊(duì)擁有眾多的化學(xué)家和生物學(xué)家，從產(chǎn)品的HNMR數(shù)據(jù)解析到生物活性數(shù)據(jù)，從客戶的詢價(jià)到產(chǎn)品的售后服務(wù)，MCE擁有著完善的管理體系，從而保證我們的服務(wù)更加高效、準(zhǔn)確。MCE為醫(yī)藥行業(yè)的發(fā)展注入了新的活力，我們是您研發(fā)工作中值得信賴的伙伴！MCE致力于為客戶提供新穎齊全的高品質(zhì)小分子活性化合物。50,000多種特異...
查看更多留言咨詢
移動(dòng)站：3-Methyladenine (3-甲基腺嘌呤）
您訪問的3-Methyladenine (3-甲基腺嘌呤）產(chǎn)品信息，由杭州昊鑫生物科技股份有限公司自行發(fā)布提供，產(chǎn)品價(jià)格為面議，如您想了解更多關(guān)于3-Methyladenine (3-甲基腺嘌呤）型號(hào)、參數(shù)、用途等信息，歡迎咨詢。
該內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由發(fā)布會(huì)員負(fù)責(zé)，為保障安全，建議您在購(gòu)買相關(guān)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)以及產(chǎn)品質(zhì)量！
相關(guān)分類
該廠商其他產(chǎn)品
相關(guān)技術(shù)文章
隨時(shí)掌握行業(yè)動(dòng)態(tài)
采購(gòu)中心
采購(gòu)專員1對(duì)1咨詢
*您想獲取產(chǎn)品的資料：
個(gè)人信息：
中方县|
当阳市|
福安市|
东至县|
温泉县|
冕宁县|
称多县|
咸阳市|
龙海市|
加查县|
文成县|
宁夏|
连州市|
崇仁县|
永和县|
宁远县|
大洼县|
汝州市|
蓝田县|
彰化市|
东山县|
睢宁县|
揭西县|
荥阳市|
贵溪市|
泰宁县|
龙岩市|
微博|
宁武县|
定州市|
关岭|
和龙市|
鄂尔多斯市|
南丰县|
长子县|
游戏|
同德县|
阳江市|
乌兰浩特市|
彭阳县|
新巴尔虎左旗|
用戶評(píng)論
發(fā)布評(píng)論