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制藥網(wǎng)>產(chǎn)品展廳>原料藥中間體、制劑>生物試劑>其它試劑>HY-D1056 Lipopolysaccharides試劑

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HY-D1056 Lipopolysaccharides試劑

參考價(jià)	￥1320.00-￥4800.00

參考價(jià)

￥1320.00-￥4800.00

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品牌 MCE: 廠商性質(zhì)
代理商

型號(hào) HY-D1056: 訪問次數(shù)
669

產(chǎn)地 浙江杭州市: 更新時(shí)間
2023/5/25 15:08:11

規(guī)格	5 mg	1320元	10000 件可售
規(guī)格	10 mg	2200元	10000 件可售
規(guī)格	25 mg	4800元	10000 件可售

Lipopolysaccharides 脂多糖 LPS

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產(chǎn)品簡介

Lipopolysaccharides試劑產(chǎn)品參數(shù)

產(chǎn)地

進(jìn)口

級(jí)別

其他

Lipopolysaccharides試劑脂多糖是從革蘭氏陰性菌外膜的外葉中提取的內(nèi)毒素，由一個(gè)抗原 O- 特異性鏈、一個(gè)核心寡糖和脂質(zhì) A 構(gòu)成。Lipopolysaccharides 是一種激活免疫系統(tǒng)的致病相關(guān)分子模式 (PAMP)。Lipopolysaccharides 激活免疫細(xì)胞的 TLR-4。本產(chǎn)品來源于大腸桿菌 O55:B5

Lipopolysaccharides試劑詳細(xì)信息

注：本產(chǎn)品僅用于科研，不可用于臨床

Lipopolysaccharides試劑生物活性

ipopolysaccharides (LPS)是一種內(nèi)毒素來源于外層膜的外膜革蘭氏陰性細(xì)菌。脂多糖由抗原O-specific鏈,一個(gè)核心的低聚糖和脂質(zhì)a脂多糖是一種病原相關(guān)分子模式(PAMP時(shí)),激活免疫系統(tǒng)。脂多糖對(duì)免疫細(xì)胞激活地[1][2][3]。這個(gè)產(chǎn)品來源于大腸桿菌O55: B5。脂多糖誘導(dǎo)的分泌細(xì)胞migrasome

Lipopolysaccharides試劑 IC₅₀ & Target

TLR4

體外研究
(In Vitro)

Lipopolysaccharides (10–80 μg/mL) selectively decreases THir (tyrosine hydroxylase immunoreactive) cells and increases culture media levels of interleukin1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as nitrite (an index of nitric oxide (NO) production)^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Lipopolysaccharides (1.5 mg/kg; i.p.; once) induces sickness and hypothermia in mice, and induces a greater and more prolonged sickness response in adult male mice^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female and male CD1 mice^[3]
Dosage:	1.5mg/kg
Administration:	Intraperitoneal injection, once
Result:	Induced sickness behavior in all mice, but adult mice displayed more sickness than pubertal mice and adult males remained sick for a longer period of time than adult females. Caused a decrease in body temperature for all mice, but this decrease was greatest in adult males. Increased pro- and anti-inflammatory cytokines at various levels in pubertal and adult male and female mice, resulted in age and sex differences in cytokine concentrations following immune challenge. Only adult males and females treated with LPS displayed significantly more IL-6 than their saline controls, and pubertal males and females and adult females displayed significantly more IL-10 than their saline controls. All the mice displayed significantly more IL-12 and TNF-α than their saline controls.

性狀

Solid

中文名稱

脂多糖

結(jié)構(gòu)分類

Saccharides

初始來源

surface of Gram-negative bacteria

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

In Vitro:

H₂O : 5 mg/mL (Need ultrasonic)

In Vivo:

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨?。以下溶解方案都?qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： PBS
Solubility: 8.33 mg/mL (Infinity mM); Clear solution; Need ultrasonic and warming and heat to 60°C

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