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染色示例	Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Stain cells with H2DCFDA. 2. Capture staining cells by spinning-disk confocal microscopy (every 5 min for 6 h). Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Remove the cell culture medium and replaced by fresh medium containing H2DCFDA and incubated with cultured cells for 30 min. 2. Wash cells with PBS for three times. 3. Use a confocal laser scanning microscopy (FV1000, Olympus Company, Japan) to detect the intracellular ROS generation. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Testing cells are seeded into a 6-well microplate at a density of 10⁵/well and cultured overnight at 37°C in 5% CO₂. Fresh medium with 50 μg/mL of different materials is added to each well and cultured for 16 h. 2. Wash cells with PBS and stain cells with H2DCFDA (20 μM; 1 h; 37°C; dark). 3. Cells are trypsinized, washed, resuspended in PBS (0.2 mL), analyzed by a flow cytometer and use a confocal laser scanning microscopy (FV1000, Olympus, Tokyo, Japan) for image. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Wash cultured cells with PBS for three times. 2. Treat testing cells with H2DCFDA in culture medium for 0.5 h in a cell incubator. 3. Use a confocal laser scanning microscopy (Olympus, FV3000) for image. Cells are analyzed on a BD LSRI Fortessa flow cytometer analyzer to quantify results. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining.

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規(guī)格	10 mM * 1 mL in DMSO	550元	10000 支可售
規(guī)格	10 mg	250元	10000 支可售
規(guī)格	25 mg	370元	10000 支可售
規(guī)格	50 mg	500元	10000 支可售
規(guī)格	100 mg	700元	10000 支可售
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HY-D0940 H2DCFDA試劑

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