英文名稱(chēng)：Hotstart Taq DNA Polymerase
其他名稱(chēng)：Hotstart Taq DNA聚合酶
分子量：94kDa，單體
級(jí)別：BR
純度：≥99%
濃度：5u/ul 
活性定義：在74℃、30分鐘內(nèi)，使10nmol脫氧核糖核酸合成滲入多聚核苷酸（吸附在DE-81上）所需的酶量
酶活性分析混合物：25mM TAPS(PH9.3,25℃), 50mM KC1, 2mM MgCL2, 0.2mM各種dATP,dGTP,dTTP, 0.1mM dCTP, 0.75mM活化的鮭魚(yú)精DNA和0.4MBq/ML(3H).-dTTP
保存緩沖液組分：20mM Tris-HCL(PH9.0), 1mM DTT, 0.1mM EDTA,100mM KC1, 0.5%(V/V) Tween 20，0.5%(v/v) Nonidet P40和50%(v/v)甘油
10×Hot start PCR Buffer：200mM Tris-HCL(PH8.3,25℃), 200mM KC1, 50mM (NH4)2SO4
抑制劑：陰離子去污劑（脫氧膽酸、肌氨酰和SDS的濃度分別高于0.06、0.02和0.01%時(shí)）
失活：酚/氯仿抽提
質(zhì)量控制：相關(guān)測(cè)試表明無(wú)內(nèi)切或外切脫氧核糖核酸酶、核糖核酸酶污染
功能啟動(dòng)：熱啟動(dòng)PCR
性狀(以下信息僅供參考)：液體。設(shè)計(jì)用于增強(qiáng)DNA擴(kuò)增的特異性、敏感性和產(chǎn)量。使用該酶可在室溫配制反應(yīng)體系，使用方便。它是一種化學(xué)修飾的重組Taq DNA Polymerase，蛋白分子氨基酸殘基上增加了熱不穩(wěn)定的封閉基團(tuán)。該酶在室溫下沒(méi)有活性，避免形成引物二聚體和非特異性延伸，從而增加DNA擴(kuò)增的特異性。95℃加熱4分鐘可恢復(fù)活性?；罨拿妇哂信cTaq DNA Polymerase相同的功能：催化5——3方向合成DNA，無(wú)可檢測(cè)的3——5校正外切酶活性，具有較低的5——3外切酶活性。該酶還具有脫氧核糖核酸酶轉(zhuǎn)移活性，在PCR產(chǎn)物的3-端多加11個(gè)腺嘌呤，活化前檢測(cè)不到這些活性。
用途：本品僅供科研，不得用于其它用途。(以下用途僅供參考)高產(chǎn)量擴(kuò)增復(fù)雜模板；PCR特異性高-降低錯(cuò)配效應(yīng)和減少引物二聚體的生成；增強(qiáng)的PCR敏感性；使用方便，室溫配制PCR反應(yīng)體系；擴(kuò)增產(chǎn)物具有3-dA突出末端；熱啟動(dòng)PCR；高產(chǎn)量擴(kuò)增長(zhǎng)達(dá)3kb片段；RT-PCR；特異性擴(kuò)增復(fù)雜cDNA和基因組模板；擴(kuò)增低拷貝DNA模板；實(shí)時(shí)PCR；多重PCR；制備TA克隆用PCR產(chǎn)物
保存：-20℃，保質(zhì)期1年
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