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磷酸酶抑制劑 Cocktail I

注：本產(chǎn)品僅用于科研，不可用于臨床

磷酸酶抑制劑 Cocktail I簡介

磷酸酶抑制劑Cocktail Ⅰ（100X，DMSO儲存液）可以有效的抑制堿性磷酸酶和serine/threonine磷酸酶，如：PP1、 PP2A，用于維持蛋白的磷酸化狀態(tài)。

Description
& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).

Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Publications

•Signal Transduct Target Ther. 2022 Feb 25;7(1):53. [Abstract]
•Signal Transduct Target Ther. 2021 Sep 4;6(1):333. [Abstract]
•Signal Transduct Target Ther. 2021 Aug 30;6(1):322. [Abstract]
•Nat Neurosci. 2023 Mar 27. [Abstract]
•Adv Funct Mater. 2020, 2004886.
•Mol Cell. 2022 Apr 8;S1097-2765(22)00265-9. [Abstract]
•Neuron. 2022 Jan 20;S0896-6273(21)01090-4. [Abstract]
•ACS Nano. 2020 Mar 24;14(3):3059-3074. [Abstract]
•Nat Commun. 2023 May 5;14(1):2602. [Abstract]
•Nat Commun. 2023 Apr 29;14(1):2480. [Abstract]
•Nat Commun. 2022 Oct 5;13(1):5871. [Abstract]
•Nat Commun. 2020 Aug 26;11(1):4254. [Abstract]
•Adv Sci (Weinh). 2021 Nov 17;e2103065. [Abstract]
•Hepatology. 2021 Jan;73(1):160-174. [Abstract]
•Cancer Commun (Lond). 2023 Feb 28. [Abstract]
•Acta Pharm Sin B. 28 October 2022.

Price Comparison

Storage

The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components

Cat. No.	Product Name	Package
HY-K0021-1 mL	Phosphatase Inhibitor Cocktail I (100× in DMSO)	1 mL
HY-K0021-10 mL	Phosphatase Inhibitor Cocktail I (100× in DMSO)	1 mL × 10
HY-K0021-50 mL	Phosphatase Inhibitor Cocktail I (100× in DMSO)	1 mL × 50
HY-K0021-100 mL	Phosphatase Inhibitor Cocktail I (100× in DMSO)	1 mL × 100