蛋白名稱:IL3Ra小鼠重組蛋白,50810-M08H
蛋白構(gòu)建:A DNA sequence encoding the mouse Il3ra (EDL20630.1) (Met1-Lys331) was expressed with a polyhistidine tag at the C-terminus.
表達(dá)宿主:HEK293 Cells
蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:
1.Loaded Recombinant Mouse IL-3R alpha/CD123 Protein, His Tag (Cat. No. 50810-M08H) on NTA Biosensor, can bind Recombinant Mouse IL-3 Protein (Cat. No. 51066-MNAH) with an affinity constant of 0.382 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
2.Recombinant Mouse IL-3R alpha/CD123 Protein, His Tag (Cat. No. 50810-M08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Mouse IL-3 Protein (Cat. No. 51066-MNAH) with an affinity constant of 2.202 μM as determined in an SPR assay (Biacore T200) (QC tested).
蛋白內(nèi)毒素:< 1.0 EU per μg protein as determined by the LAL method.
預(yù)測N端:Ser 17
蛋白分子量:The recombinant mouse Il3ra consists 326 amino acids and predicts a molecular mass of 35.9 kDa.
蛋白NP號(hào):EDL20630.1
蛋白氨基酸序列:Met1-Lys331
蛋白標(biāo)簽:C-His
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