測定方法  比色法（570nm）或熒光法（585nm/530nm）定量測定氨/銨離子的濃度。

產(chǎn)品說明：丙酮酸在細胞代謝中起關鍵作用。丙酮酸可以通過把葡萄糖轉化為碳水化合物，通過乙酰輔酶A轉化為脂肪或能量，也可轉化為丙氨酸和乙醇。人體內丙酮酸的非正常水平與肝臟疾病和代謝疾病有關。簡單、直接、自動化測量丙酮酸濃度的手段，在研究和藥物研發(fā)中受到廣泛的應用。我公司生產(chǎn)的丙酮酸測試方法使用單一工作試劑將丙酮酸氧化和*的檢測一次性完成。在波長為570nm檢測的顏色強度或 lem/ex = 585/530nm處的熒光強度與樣品中丙酮酸濃度成正比。

產(chǎn)品特點   靈敏、準確：只需10 mL樣本。96孔板中檢測范圍：比色法2 - 500 mM， 熒光法0.2 - 50 mM丙酮酸。簡單、方便： 該過程包含了添加單一工作試劑，在溫室下培養(yǎng)30分鐘。改良了試劑的穩(wěn)定性：優(yōu)化的配方，大大增加了試劑和信號穩(wěn)定。

產(chǎn)品用途:   可直接檢測生物樣品中的丙酮酸。用于藥品和藥理學研究，檢測藥物對丙酮酸代謝的影響。

儲存條件： 冰運，在 -20°C下儲藏。

Description   Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. our pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample.

Key features    Sensitive and accurate. Use as little as 10 mL samples. Linear detection range in 96-well plate: 2 to 500 mM (17 mg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 mM for fluorimetric assays.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Applications:   Direct Assays: pyruvate in biological samples.Drug Discovery/Pharmacology: effects of drugs on pyruvate metabolism.

Storage conditions. The kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of three months after receipt.

編號：EPYR-048，EPYR-100

測試：48T,100T