說明書下載EADP.rar

分類名稱 : 代謝產(chǎn)物分析 產(chǎn)品介紹 :

產(chǎn)品說明  本公司研制的ADP檢測試劑盒可以快速檢測細胞ADP含量。檢測共分兩步，首先用工作試劑將細胞溶解，釋放出ATP和ADP。在熒光素酶的作用下，ATP可與D-蟲熒光素快速反應(yīng)并發(fā)光，其光強度可以作為細胞間ATP濃度的直接衡量標(biāo)準(zhǔn)。然后，ADP通過酶化反應(yīng)轉(zhuǎn)化為ATP。而新生成的ATP再次以同樣方式與D-蟲熒光素發(fā)生反應(yīng)。本產(chǎn)品通過試劑配方的優(yōu)化，大大提高了光信號的穩(wěn)定性。檢測中，初次ATP檢測所產(chǎn)生的光量將始終保持穩(wěn)定。因此，第二次光強度檢測可表征樣本中總的ADP與ATP濃度。該檢測無放射性，可用微板進行操作，試劑適用于所有培養(yǎng)液。在利用96或384孔板進行高通量篩選時，試劑適用于所有液體操作系統(tǒng)。

產(chǎn)品特點  安全：檢測無放射性。測量敏感、精確：可測定zui低0.1μM的ADP。操作簡便：“混合-反應(yīng)-測量”型檢測，無需清洗及轉(zhuǎn)移試劑操作。高通量：在96孔板和384孔板檢測中，Z’因子大于0.5。通過高通量液體操作系統(tǒng)，每天可檢測上千份樣本。

產(chǎn)品用途  可用于細胞中或其他生物樣本中ADP含量的檢測。

儲存條件  所有試劑于-20℃下保存。冰運。

DESCRIPTION  Our ADP Assay Kit provides a rapid method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediay reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. Due to a special formulation of the reagent system which greatly stabilizes the light signal generated by the luciferase reaction, the luminescence from the initial ATP measurement remains stable throughout this assay. Therefore, the second light intensity measured represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

KEY FEATURES  Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 μM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

APPLICATIONS  ADP determination in cells and other biological samples.

STORAGE CONDITIONS  store all reagents at -20°C. This product is shipped on ice. Shelf life of at least 6 months.

編號：EADP-048，EADP-100

測試：48T,100T