說明書下載DIOX.rar

分類名稱 : 氧化應(yīng)激測定,代謝產(chǎn)物分析 產(chǎn)品介紹 :

測定方法  比色法（585nm）定量測定過氧化物的濃度。

產(chǎn)品說明  過氧化物（如*）是在氧化應(yīng)激條件下形成的一種重要的活性氧。過氧化物含量高可能與衰老、哮喘、糖尿病、動(dòng)脈漿樣硬化、白內(nèi)障、關(guān)節(jié)炎和神經(jīng)組織退化等疾病有關(guān)。在科研和藥物研發(fā)中，方便、直接、自動(dòng)化的過氧化物含量檢測手段是zui為理想的。本公司研制的過氧化物檢測試劑盒可直接用于生物樣品的檢測，免除了預(yù)處理過程。檢測原理是利用試劑與過氧化物氧化反應(yīng)生成紫色復(fù)合物的特性，在波長540-610nm (585nm) 下檢測，其顏色深淺程度與樣品中過氧化物的含量成正比。

產(chǎn)品特點(diǎn)  敏感、準(zhǔn)確：用山梨醇加深顏色，在96孔板中測試，雙氧水的檢測范圍在0.4-100 μM（14-3400 ng/mL）。 簡單、高通量：整個(gè)測試過程只需加入一種工作試劑并反應(yīng)30分鐘。利用96孔板的高通量檢測手段，每天可以測試上千份樣本。

產(chǎn)品用途  1．直接檢測生物樣本中過氧化物含量（如，血清、血漿、尿液、細(xì)胞溶液和培養(yǎng)液。 2．適用于藥理學(xué)研究，檢測藥物對過氧化物代謝的影響。

儲(chǔ)藏條件  可在室溫下運(yùn)輸。試劑在4℃環(huán)境下保存.

DESCRIPTION  Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases. Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. Our peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes a chromogenic reagent, which forms a purple complex with peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample.

KEY FEATURES  Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.4 μM (14 ng/mL) to 100 μM (3,400 ng/mL) H2O2 in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

APPLICATIONS  Direct Assays: H2O2 in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium). Pharmacology: effects of drugs on peroxide metabolism.

STORAGE CONDITIONS  The kit is shipped at room temperature. Store all reagents at 4 °C. Shelf life of at least 6 months.

編號：DIOX-048，DIOX-250

測試：48T,250T