說明書下載DIUR.rar

分類名稱 : 血液尿液化學,代謝產物分析 產品介紹 :

產品說明  尿素是機體中蛋白質代謝的主要終產物，其在肝臟中產生并經由腎臟排出。通過尿素排泄，可以把有毒的氨排出體外。尿素指標對評定病人腎臟功能具有重要意義。一般來說，尿素含量升高可能意味著患有腎炎、腎缺血、尿路栓塞或某些非腎性疾病如充血性心力衰竭、肝臟疾病、糖尿病等；而尿素含量較少則有可能是急性肝功能不全或治療性體液儲留造成的。簡單﹑直接﹑高度自動化的尿素檢測手段是十分理想的。本公司的尿素檢測試劑盒可直接對生物樣品檢測，無需預處理過程。檢測基于改良的Jung法，利用顯色試劑與尿素反應生成有色復合物的特性，在520nm波長下測量，其顏色深淺與尿素濃度成正比。并且通過配方的改良，充分減小了未處理的樣品中其他雜質的干擾。

產品特點  測量敏感、準確：在96孔板中檢測僅需5μL樣品，尿素線性測試范圍在0.08-100 mg/dL (0.013-17mM)。簡單、高通量：整個測試過程只需加入試劑并培養(yǎng)20分鐘。通過96孔板上的高通量檢測手段，每天可以測試上千個樣品。試劑穩(wěn)定性高和多功能性：產品經過配方的改良，*的提高了試劑和測試信號的穩(wěn)定性，測試可采用比色皿或96孔板。受樣品干擾小：樣品無需預處理，不受氨影響，可以直接檢測生物樣品。

產品用途  1．直接檢測血清、血漿、尿液、乳汁、細胞/組織培養(yǎng)物和支氣管肺泡液等生物樣品中的尿素含量。2．用于藥品和藥理學研究，檢測藥物對尿素代謝的影響。3. 可用于檢測污水和土壤中尿素的含量。

儲藏條件  在室溫下運輸。所有試劑在2-8℃環(huán)境下保存。如需長時間存放，請將標準品放在-20℃下保存.

DESCRIPTION  Urea is primarily produced in the liver and secreted by the kidneys. Urea is the major end product of protein catabolism in animals. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases, e.g., congestive heart failure, liver diseases and diabetes. Decreased levels indicate acute hepatic insufficiency or may result from over-vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea concentration or blood urea nitrogen BUN in biological samples are becoming popular in Research and Drug Discovery. Our urea assay kit is designed to measure urea directly in biological samples without any pretreatment. The improved Jung method utilizes a chromogenic reagent that forms a colored complex specifically with urea. The intensity of the color, measured at 520nm, is directly proportional to the urea concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

KEY FEATURES  Sensitive and accurate. Use 5 μL samples. Linear detection range 0.08-100mg/dL (0.013-17mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the
presence of lipid and protein.

APPLICATIONS  Direct Assays: urea in serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL) etc. Drug Discovery/Pharmacology: effects of drugs on urea metabolism. Environment: urea determination in waste water, soil etc.

STORAGE CONDITIONS  keep standard at –20°C. Shelf life: at least 6 months.

編號：DIUR-048，DIUR-500

測試：48T,500T