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花菁類染料（Cyanine dyes）的分類介紹及選用指南

發(fā)布時間：2018-8-10

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花菁類染料（Cyanine dyes）的分類介紹及選用指南

花菁這類染料的分子內(nèi)部含有由甲川基(CH)n組成的共軛鏈，n可為奇數(shù)或偶數(shù)。共軛鏈兩端或鏈中間連有雜環(huán)、芳環(huán)化合物、環(huán)烯化合物等與共軛鏈組成一個大的共軛體系，分子內(nèi)部的氫可被一定數(shù)目的各類取代基取代。

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基于其結構，花菁通常有非常高的超過100000 Lmol^-1cm^-1消光系數(shù)。不同的取代基具有控制發(fā)色團的性能，如吸收波長、耐光性、熒光。例如，通過聚次甲基橋鍵長度的選擇來控制吸光度和熒光波長：聚次甲基橋鍵越長其吸光度越高且發(fā)射波長可達到近紅外區(qū)。

已經(jīng)有許多的花菁染料（Cyanine dyes）用于生命科學領域，一系列的噻唑和惡唑染料用作DNA 和蛋白質(zhì)染色(如TOTO，YOYO， Stains All和其它)。但是在生命科學研究領域的花菁染料是由卡內(nèi)基梅隆大學的Alan Waggoner和他的同事們在1990年代早期發(fā)現(xiàn)的。該花菁染料是經(jīng)過修飾后的吲哚菁綠(ICG)，從1970年代以來該染料一直用于血管造影術，而且他們都包含兩個假吲哚環(huán)側翼(CH)n鏈。發(fā)現(xiàn)此類染料對生物分子表現(xiàn)出較低的非特異性結合，由于巨大的消光系數(shù)能發(fā)出明亮的熒光和良好的量子產(chǎn)量。一旦獲得，在卡內(nèi)基梅隆過期后這些分子可在公共領域獲得，并從Lumiprobe可購買到，作為各種反應衍生物用于科研和商業(yè)，比如用于點擊化學的NHS酯、馬來酰亞胺、疊氮化物的衍生品和其他衍生物。

花菁染料分為兩種：非磺化的花菁染料和磺化的花菁染料，對于許多的應用，他們是可以互相替換的，因為他們的光譜屬性幾乎相同?；腔头腔腔玖峡捎糜贒NA和蛋白質(zhì)等生物分子的標記。兩種染料的區(qū)別在于它們的溶解度：磺基染料是水溶性，他們可在水環(huán)境中標記無需添加有機助溶劑，他們在水里的分散性較好。在某些情況下，你可能會只需要這兩種染料中的一種（下面將具體講述非磺化的花菁染料和磺化的花菁染料的具體區(qū)別。）

非磺化的花菁染料

可用的非磺化的花菁染料包括Cy3，Cy3.5，Cy5，Cy5.5，Cy7，和Cy7.5，Cy代表‘cyanine’，*個數(shù)字代表了假吲哚之間的碳原子數(shù)。Cy2是惡唑衍生物而不是假吲哚，是不遵守這個規(guī)則的。后綴.5是苯丙稠合的花菁染料。結構的變化也能改變分子的熒光特性，及覆蓋zui重要的可見光和攜帶幾個熒光基團的近紅外光譜。

大部分非磺化的花菁染料衍生物（酰肼鹽酸鹽類和胺類除外）具有低水溶性，當這些分子用于生物分子標記時，使用有機溶劑作為助溶劑(5-20%的DMF或DMSO)是有效的反應所必需的。花菁染料應先溶解在有機溶劑中，之后在適當?shù)乃芫彌_劑作用下加入到生物分子溶液中（蛋白質(zhì)，肽，氨基標記的 DNA）。在染料沉淀之前，染料分子與生物分子發(fā)生有效反應。

非磺化的花菁染料的熒光性質(zhì)對溶劑和反應環(huán)境的依賴性較小，非磺化的的花菁染料的吸光度和熒光光譜繪制如下。

磺化的花菁染料

磺化的花菁染料包含了額外地磺基官能團能促進染料分子在水相中的溶解度，帶電荷的磺酸鹽降低了染料分子的聚合度且能很好地標記偶聯(lián)物。

目前可用的磺化花菁染料包括sulfo-Cy3，sulfo-Cy5和sulfo-Cy7。

磺化花菁染料具有高度水溶性。標記生物分子時不需要有機助溶劑輔助。

磺化和非磺化花菁染料之間的異同

磺化和非磺化花菁染料都展現(xiàn)出非常相似的熒光性能。然而，在使用說明上有幾個不同點需要注意，非磺化花菁染料必須要先溶解在有機助溶劑中（DMF or DMSO），之后在適當?shù)乃芫彌_劑作用下加入靶標生物分子溶液。推薦使用的助溶劑的體積為，溶解Cy3， Cy5， Cy7使用10%助溶劑，.5的染料使用15%的助溶劑。而磺化的花菁染料可以在純粹的水環(huán)境中應用。關于花菁染料在純化方面的差異：當使用與水或水溶緩沖溶液相關的透析純化染料時，必須使用sulfo-Cy以實現(xiàn)除去未反應的染料材料。與磺化和非磺化花菁染料的反應可以通過凝膠過濾，色譜法 (FPLC，液相色譜法，離子交換法)，或電泳純化。

磺化和非磺化花菁染料在標記以下類別目標時是可互換的:

1.可溶性蛋白，其對額外添加的有機助溶劑具有耐受性

2.抗體(使用5 - 10% DMSO / DMF)

3.DNA和寡核苷酸

4.肽

5.許多小分子

注：用類似磺化和非磺化的試劑標記的共軛物（例如：sulfo-Cy5 和Cy5）的熒光性質(zhì)是非常相似的，可用于各種熒光儀器。

以下情況必須選用磺化花菁染料：

1.遇DMF 或 DMSO變性的敏感蛋白質(zhì)

2.通過透析純化的蛋白質(zhì)偶聯(lián)物

3.在水溶液中有納米粒子

4.不溶于水或疏水的蛋白

以下情況必須選用非磺化花菁染料：

在有機相中發(fā)生的反應（二氯甲烷，腈化甲烷）

西安凱新生物科技有限公司花菁染料類染料列表：

脂溶性花菁染料：

Cyanine2 NHS ester minimal dye, 5 nmol

Cyanine2 NHS ester minimal dye, 10 nmol

Cyanine2 NHS ester minimal dye, 25 nmol

Cyanine3 alkyne, 1 mg

Cyanine3 alkyne, 5 mg

Cyanine3 alkyne, 10 mg

Cyanine3 alkyne, 25 mg

Cyanine3 alkyne, 50 mg

Cyanine3 alkyne, 100 mg

Cyanine3 amine, 1 mg

Cyanine3 amine, 5 mg

Cyanine3 amine, 25 mg

Cyanine3 amine, 50 mg

Cyanine3 amine, 100 mg

Cyanine3 azide, 100 uL, 10 mM/DMSO

Cyanine3 azide, 500 uL, 10 mM/DMSO

Cyanine3 azide, 1 mL, 10 mM/DMSO

Cyanine3 azide, 1 mg

Cyanine3 azide, 5 mg

Cyanine3 azide, 10 mg

Cyanine3 azide, 25 mg

Cyanine3 azide, 50 mg

Cyanine3 azide, 100 mg

Cyanine3 carboxylic acid, 1 mg

Cyanine3 carboxylic acid, 5 mg

Cyanine3 carboxylic acid, 25 mg

Cyanine3 carboxylic acid, 50 mg

Cyanine3 carboxylic acid, 100 mg

Cyanine3 DBCO, 1 mg

Cyanine3 DBCO, 5 mg

Cyanine3 DBCO, 10 mg

Cyanine3 DBCO, 25 mg

Cyanine3 DBCO, 50 mg

Cyanine3 DBCO, 100 mg

Cyanine3 hydrazide, 1 mg

Cyanine3 hydrazide, 5 mg

Cyanine3 hydrazide, 25 mg

Cyanine3 hydrazide, 50 mg

Cyanine3 hydrazide, 100 mg

Cyanine3 maleimide, 1 mg

Cyanine3 maleimide, 5 mg

Cyanine3 maleimide, 25 mg

Cyanine3 maleimide, 50 mg

Cyanine3 maleimide, 100 mg

Cyanine3 NHS ester, 5 mg

Cyanine3 NHS ester, 25 mg

Cyanine3 NHS ester, 50 mg

Cyanine3 NHS ester, 100 mg

Cyanine3 NHS ester minimal dye, 5 nmol

Cyanine3 NHS ester minimal dye, 10 nmol

Cyanine3 NHS ester minimal dye, 25 nmol

Cyanine3 tetrazine, 1 mg

Cyanine3 tetrazine, 5 mg

Cyanine3 tetrazine, 25 mg

Cyanine3 tetrazine, 50 mg

Cyanine3 tetrazine, 100 mg

Cyanine3.5 azide, 100 uL, 10 mM/DMSO

Cyanine3.5 azide, 500 uL, 10 mM/DMSO

Cyanine3.5 azide, 1 mL, 10 mM/DMSO

Cyanine3.5 azide, 1 mg

Cyanine3.5 azide, 5 mg

Cyanine3.5 azide, 10 mg

Cyanine3.5 azide, 25 mg

Cyanine3.5 azide, 50 mg

Cyanine3.5 carboxylic acid, 1 mg

Cyanine3.5 carboxylic acid, 5 mg

Cyanine3.5 carboxylic acid, 25 mg

Cyanine3.5 carboxylic acid, 50 mg

Cyanine3.5 carboxylic acid, 100 mg

Cyanine3.5 NHS ester, 1 mg

Cyanine3.5 NHS ester, 5 mg

Cyanine3.5 NHS ester, 25 mg

Cyanine3.5 NHS ester, 50 mg

Cyanine3.5 NHS ester, 100 mg

Cyanine5 alkyne, 1 mg

Cyanine5 alkyne, 5 mg

Cyanine5 alkyne, 10 mg

Cyanine5 alkyne, 25 mg

Cyanine5 alkyne, 50 mg

Cyanine5 alkyne, 100 mg

Cyanine5 amine, 1 mg

Cyanine5 amine, 5 mg

Cyanine5 amine, 25 mg

Cyanine5 amine, 50 mg

Cyanine5 amine, 100 mg

Cyanine5 azide, 100 uL, 10 mM/DMSO

Cyanine5 azide, 500 uL, 10 mM/DMSO

Cyanine5 azide, 1 mL, 10 mM/DMSO

Cyanine5 azide, 1 mg

Cyanine5 azide, 5 mg

Cyanine5 azide, 10 mg

Cyanine5 azide, 25 mg

Cyanine5 azide, 50 mg

Cyanine5 azide, 100 mg

Cyanine5 Boc-hydrazide, 1 mg

Cyanine5 Boc-hydrazide, 5 mg

Cyanine5 Boc-hydrazide, 25 mg

Cyanine5 Boc-hydrazide, 50 mg

Cyanine5 Boc-hydrazide, 100 mg

Cyanine5 carboxylic acid, 1 mg

Cyanine5 carboxylic acid, 5 mg

Cyanine5 carboxylic acid, 25 mg

Cyanine5 carboxylic acid, 50 mg

Cyanine5 carboxylic acid, 100 mg

Cyanine5 DBCO, 1 mg

Cyanine5 DBCO, 5 mg

Cyanine5 DBCO, 10 mg

Cyanine5 DBCO, 25 mg

Cyanine5 DBCO, 50 mg

Cyanine5 DBCO, 100 mg

Cyanine5 hydrazide, 1 mg

Cyanine5 hydrazide, 5 mg

Cyanine5 hydrazide, 25 mg

Cyanine5 hydrazide, 50 mg

Cyanine5 hydrazide, 100 mg

Cyanine5 maleimide, 1 mg

Cyanine5 maleimide, 5 mg

Cyanine5 maleimide, 25 mg

Cyanine5 maleimide, 50 mg

Cyanine5 maleimide, 100 mg

<="" a="">Cyanine5 NHS ester, 1 mg

Cyanine5 NHS ester, 5 mg

Cyanine5 NHS ester, 25 mg

Cyanine5 NHS ester, 50 mg

Cyanine5 NHS ester, 100 mg

Cyanine5 NHS ester minimal dye, 5 nmol

Cyanine5 NHS ester minimal dye, 10 nmol

Cyanine5 NHS ester minimal dye, 25 nmol

Cyanine5 tetrazine, 1 mg

Cyanine5 tetrazine, 5 mg

Cyanine5 tetrazine, 25 mg

Cyanine5 tetrazine, 50 mg

Cyanine5 tetrazine, 100 mg

Cyanine5.5 alkyne, 1 mg

Cyanine5.5 alkyne, 5 mg

Cyanine5.5 alkyne, 10 mg

Cyanine5.5 alkyne, 25 mg

Cyanine5.5 alkyne, 50 mg

Cyanine5.5 alkyne, 100 mg

Cyanine5.5 amine, 1 mg

Cyanine5.5 amine, 5 mg

Cyanine5.5 amine, 25 mg

Cyanine5.5 amine, 50 mg

Cyanine5.5 amine, 100 mg

Cyanine5.5 azide, 100 uL, 10 mM/DMSO

Cyanine5.5 azide, 500 uL, 10 mM/DMSO

Cyanine5.5 azide, 1 mL, 10 mM/DMSO

Cyanine5.5 azide, 1 mg

Cyanine5.5 azide, 5 mg

Cyanine5.5 azide, 10 mg

Cyanine5.5 azide, 25 mg

Cyanine5.5 azide, 50 mg

Cyanine5.5 carboxylic acid, 1 mg

Cyanine5.5 carboxylic acid, 5 mg

Cyanine5.5 carboxylic acid, 25 mg

Cyanine5.5 carboxylic acid, 50 mg

Cyanine5.5 carboxylic acid, 100 mg

Cyanine5.5 hydrazide, 1 mg

Cyanine5.5 hydrazide, 5 mg

Cyanine5.5 hydrazide, 25 mg

Cyanine5.5 hydrazide, 50 mg

Cyanine5.5 hydrazide, 100 mg

Cyanine5.5 maleimide, 1 mg

Cyanine5.5 maleimide, 5 mg

Cyanine5.5 maleimide, 25 mg

Cyanine5.5 maleimide, 50 mg

Cyanine5.5 maleimide, 100 mg

Cyanine5.5 NHS ester, 1 mg

Cyanine5.5 NHS ester, 5 mg

Cyanine5.5 NHS ester, 25 mg

Cyanine5.5 NHS ester, 50 mg

Cyanine5.5 NHS ester, 100 mg

Cyanine5.5 tetrazine, 1 mg

Cyanine5.5 tetrazine, 5 mg

Cyanine5.5 tetrazine, 25 mg

Cyanine5.5 tetrazine, 50 mg

Cyanine5.5 tetrazine, 100 mg

Cyanine7 alkyne, 1 mg

Cyanine7 alkyne, 5 mg

Cyanine7 alkyne, 10 mg

Cyanine7 alkyne, 25 mg

Cyanine7 alkyne, 50 mg

Cyanine7 alkyne, 100 mg

Cyanine7 amine, 1 mg

Cyanine7 amine, 5 mg

Cyanine7 amine, 25 mg

Cyanine7 amine, 50 mg

Cyanine7 amine, 100 mg

Cyanine7 azide, 100 uL, 10 mM/DMSO

Cyanine7 azide, 500 uL, 10 mM/DMSO

Cyanine7 azide, 1 mL, 10 mM/DMSO

Cyanine7 azide, 1 mg

Cyanine7 azide, 5 mg

Cyanine7 azide, 10 mg

Cyanine7 azide, 25 mg

Cyanine7 azide, 50 mg

Cyanine7 carboxylic acid, 1 mg

Cyanine7 carboxylic acid, 5 mg

Cyanine7 carboxylic acid, 25 mg

Cyanine7 carboxylic acid, 50 mg

Cyanine7 carboxylic acid, 100 mg

Cyanine7 DBCO, 1 mg

Cyanine7 DBCO, 5 mg

Cyanine7 DBCO, 10 mg

Cyanine7 DBCO, 25 mg

Cyanine7 DBCO, 50 mg

Cyanine7 DBCO, 100 mg

Cyanine7 dicarboxylic acid, 100 mg

Cyanine7 dicarboxylic acid, 1 mg

Cyanine7 dicarboxylic acid, 25 mg

Cyanine7 dicarboxylic acid, 50 mg

Cyanine7 dicarboxylic acid, 5 mg

Cyanine7 hydrazide, 1 mg

Cyanine7 hydrazide, 5 mg

Cyanine7 hydrazide, 25 mg

Cyanine7 hydrazide, 50 mg

Cyanine7 hydrazide, 100 mg

Cyanine7 maleimide, 1 mg

Cyanine7 maleimide, 5 mg

Cyanine7 maleimide, 25 mg

Cyanine7 maleimide, 50 mg

Cyanine7 maleimide, 100 mg

Cyanine7 NHS ester, 1 mg

Cyanine7 NHS ester, 5 mg

Cyanine7 NHS ester, 25 mg

Cyanine7 NHS ester, 50 mg

Cyanine7 NHS ester, 100 mg

Cyanine7 tetrazine, 1 mg

Cyanine7 tetrazine, 5 mg

Cyanine7 tetrazine, 25 mg

Cyanine7 tetrazine, 50 mg

Cyanine7 tetrazine, 100 mg

Cyanine7.5 alkyne, 1 mg

Cyanine7.5 alkyne, 5 mg

Cyanine7.5 alkyne, 10 mg

Cyanine7.5 alkyne, 25 mg

Cyanine7.5 alkyne, 50 mg

Cyanine7.5 alkyne, 100 mg

Cyanine7.5 amine, 1 mg

Cyanine7.5 amine, 5 mg

Cyanine7.5 amine, 10 mg

Cyanine7.5 amine, 25 mg

Cyanine7.5 amine, 50 mg

Cyanine7.5 amine, 100 mg

Cyanine7.5 azide, 100 uL, 10 mM/DMSO

Cyanine7.5 azide, 500 uL, 10 mM/DMSO

Cyanine7.5 azide, 1 mL, 10 mM in DMSO

Cyanine7.5 azide, 1 mg

Cyanine7.5 azide, 5 mg

Cyanine7.5 azide, 10 mg

Cyanine7.5 azide, 25 mg

Cyanine7.5 azide, 50 mg

Cyanine7.5 azide, 100 mg

Cyanine7.5 carboxylic acid, 1 mg

Cyanine7.5 carboxylic acid, 5 mg

Cyanine7.5 carboxylic acid, 25 mg

Cyanine7.5 carboxylic acid, 50 mg

Cyanine7.5 carboxylic acid, 100 mg

Cyanine7.5 hydrazide, 1 mg

Cyanine7.5 hydrazide, 5 mg

Cyanine7.5 hydrazide, 25 mg

Cyanine7.5 hydrazide, 50 mg

Cyanine7.5 hydrazide, 100 mg

Cyanine7.5 maleimide, 1 mg

Cyanine7.5 maleimide, 5 mg

Cyanine7.5 maleimide, 25 mg

Cyanine7.5 maleimide, 50 mg

Cyanine7.5 maleimide, 100 mg

Cyanine7.5 NHS ester, 5 mg

Cyanine7.5 NHS ester, 25 mg

Cyanine7.5 NHS ester, 50 mg

Cyanine7.5 NHS ester, 100 mg

Cyanine7.5 tetrazine, 1 mg

Cyanine7.5 tetrazine, 5 mg

Cyanine7.5 tetrazine, 25 mg

Cyanine7.5 tetrazine, 50 mg

Cyanine7.5 tetrazine, 100 mg

水溶性花菁染料：

Sulfo-Cyanine3 alkyne, 1 mg

Sulfo-Cyanine3 alkyne, 5 mg

Sulfo-Cyanine3 alkyne, 10 mg

Sulfo-Cyanine3 alkyne, 25 mg

Sulfo-Cyanine3 alkyne, 50 mg

Sulfo-Cyanine3 alkyne, 100 mg

Sulfo-Cyanine3 amine, 1 mg

Sulfo-Cyanine3 amine, 5 mg

Sulfo-Cyanine3 amine, 25 mg

Sulfo-Cyanine3 amine, 50 mg

Sulfo-Cyanine3 amine, 100 mg

Sulfo-Cyanine3 azide, 1 mg

Sulfo-Cyanine3 azide, 5 mg

Sulfo-Cyanine3 azide, 10 mg

Sulfo-Cyanine3 azide, 25 mg

Sulfo-Cyanine3 azide, 50 mg

Sulfo-Cyanine3 azide, 100 mg

Sulfo-Cyanine3 carboxylic acid, 1 mg

Sulfo-Cyanine3 carboxylic acid, 5 mg

Sulfo-Cyanine3 carboxylic acid, 25 mg

Sulfo-Cyanine3 carboxylic acid, 50 mg

Sulfo-Cyanine3 carboxylic acid, 100 mg

Sulfo-Cyanine3 maleimide, 1 mg

Sulfo-Cyanine3 maleimide, 5 mg

Sulfo-Cyanine3 maleimide, 25 mg

Sulfo-Cyanine3 maleimide, 50 mg

Sulfo-Cyanine3 maleimide, 100 mg

Sulfo-Cyanine3 NHS ester, 1 mg

Sulfo-Cyanine3 NHS ester, 5 mg

Sulfo-Cyanine3 NHS ester, 25 mg

Sulfo-Cyanine3 NHS ester, 50 mg

Sulfo-Cyanine3 NHS ester, 100 mg

Sulfo-Cyanine3 tetrazine, 1 mg

Sulfo-Cyanine3 tetrazine, 5 mg

Sulfo-Cyanine3 tetrazine, 10 mg

Sulfo-Cyanine3 tetrazine, 25 mg

Sulfo-Cyanine3 tetrazine, 50 mg

Sulfo-Cyanine3 tetrazine, 100 mg

Sulfo-Cyanine5 alkyne, 1 mg

Sulfo-Cyanine5 alkyne, 5 mg

Sulfo-Cyanine5 alkyne, 10 mg

Sulfo-Cyanine5 alkyne, 25 mg

Sulfo-Cyanine5 alkyne, 50 mg

Sulfo-Cyanine5 alkyne, 100 mg

Sulfo-Cyanine5 amine, 1 mg

Sulfo-Cyanine5 amine, 5 mg

Sulfo-Cyanine5 amine, 25 mg

Sulfo-Cyanine5 amine, 50 mg

Sulfo-Cyanine5 amine, 100 mg

Sulfo-Cyanine5 azide, 1 mg

Sulfo-Cyanine5 azide, 5 mg

Sulfo-Cyanine5 azide, 10 mg

Sulfo-Cyanine5 azide, 25 mg

Sulfo-Cyanine5 azide, 50 mg

Sulfo-Cyanine5 azide, 100 mg

Sulfo-Cyanine5 bis-NHS ester, 1 mg

Sulfo-Cyanine5 bis-NHS ester, 5 mg

Sulfo-Cyanine5 bis-NHS ester, 25 mg

Sulfo-Cyanine5 bis-NHS ester, 50 mg

Sulfo-Cyanine5 bis-NHS ester, 100 mg

Sulfo-Cyanine5 carboxylic acid, 1 mg

Sulfo-Cyanine5 carboxylic acid, 5 mg

Sulfo-Cyanine5 carboxylic acid, 25 mg

Sulfo-Cyanine5 carboxylic acid, 50 mg

Sulfo-Cyanine5 carboxylic acid, 100 mg

Sulfo-Cyanine5 dUTP, 250 nmol

Sulfo-Cyanine5 dUTP, 50 nmol

Sulfo-Cyanine5 maleimide, 1 mg

Sulfo-Cyanine5 maleimide, 5 mg

Sulfo-Cyanine5 maleimide, 25 mg

Sulfo-Cyanine5 maleimide, 50 mg

Sulfo-Cyanine5 maleimide, 100 mg

Sulfo-Cyanine5 NHS ester, 1 mg

Sulfo-Cyanine5 NHS ester, 5 mg

Sulfo-Cyanine5 NHS ester, 25 mg

Sulfo-Cyanine5 NHS ester, 50 mg

Sulfo-Cyanine5 NHS ester, 100 mg

Sulfo-Cyanine5 tetrazine, 1 mg

Sulfo-Cyanine5 tetrazine, 5 mg

Sulfo-Cyanine5 tetrazine, 10 mg

Sulfo-Cyanine5 tetrazine, 25 mg

Sulfo-Cyanine5 tetrazine, 50 mg

Sulfo-Cyanine5 tetrazine, 100 mg

Sulfo-Cyanine5.5 alkyne, 1 mg

Sulfo-Cyanine5.5 alkyne, 5 mg

Sulfo-Cyanine5.5 alkyne, 10 mg

Sulfo-Cyanine5.5 alkyne, 25 mg

Sulfo-Cyanine5.5 alkyne, 50 mg

Sulfo-Cyanine5.5 alkyne, 100 mg

Sulfo-Cyanine5.5 amine, 1 mg

Sulfo-Cyanine5.5 amine, 5 mg

Sulfo-Cyanine5.5 amine, 25 mg

Sulfo-Cyanine5.5 amine, 50 mg

Sulfo-Cyanine5.5 amine, 100 mg

Sulfo-Cyanine5.5 azide, 1 mg

Sulfo-Cyanine5.5 azide, 5 mg

Sulfo-Cyanine5.5 azide, 10 mg

Sulfo-Cyanine5.5 azide, 25 mg

Sulfo-Cyanine5.5 azide, 50 mg

Sulfo-Cyanine5.5 azide, 100 mg

Sulfo-Cyanine5.5 carboxylic acid, 1 mg

Sulfo-Cyanine5.5 carboxylic acid, 5 mg

Sulfo-Cyanine5.5 carboxylic acid, 25 mg

Sulfo-Cyanine5.5 carboxylic acid, 50 mg

Sulfo-Cyanine5.5 carboxylic acid, 100 mg

Sulfo-Cyanine5.5 maleimide, 1 mg

Sulfo-Cyanine5.5 maleimide, 5 mg

Sulfo-Cyanine5.5 maleimide, 25 mg

Sulfo-Cyanine5.5 maleimide, 50 mg

Sulfo-Cyanine5.5 maleimide, 100 mg

Sulfo-Cyanine5.5 NHS ester, 1 mg

Sulfo-Cyanine5.5 NHS ester, 5 mg

Sulfo-Cyanine5.5 NHS ester, 25 mg

Sulfo-Cyanine5.5 NHS ester, 50 mg

Sulfo-Cyanine5.5 NHS ester, 100 mg

Sulfo-Cyanine7 alkyne, 1 mg

Sulfo-Cyanine7 alkyne, 5 mg

Sulfo-Cyanine7 alkyne, 10 mg

Sulfo-Cyanine7 alkyne, 25 mg

Sulfo-Cyanine7 alkyne, 50 mg

Sulfo-Cyanine7 alkyne, 100 mg

Sulfo-Cyanine7 amine, 1 mg

Sulfo-Cyanine7 amine, 5 mg

Sulfo-Cyanine7 amine, 25 mg

Sulfo-Cyanine7 amine, 50 mg

Sulfo-Cyanine7 amine, 100 mg

Sulfo-Cyanine7 azide, 1 mg

Sulfo-Cyanine7 azide, 5 mg

Sulfo-Cyanine7 azide, 10 mg

Sulfo-Cyanine7 azide, 25 mg

Sulfo-Cyanine7 azide, 50 mg

Sulfo-Cyanine7 azide, 100 mg

Sulfo-Cyanine7 carboxylic acid, 1 mg

Sulfo-Cyanine7 carboxylic acid, 5 mg

Sulfo-Cyanine7 carboxylic acid, 25 mg

Sulfo-Cyanine7 carboxylic acid, 50 mg

Sulfo-Cyanine7 carboxylic acid, 100 mg

sulfo-Cyanine7 dicarboxylic acid, 100 mg

sulfo-Cyanine7 dicarboxylic acid, 1 mg

sulfo-Cyanine7 dicarboxylic acid, 25 mg

sulfo-Cyanine7 dicarboxylic acid, 50 mg

sulfo-Cyanine7 dicarboxylic acid, 5 mg

Sulfo-Cyanine7 maleimide, 1 mg

Sulfo-Cyanine7 maleimide, 5 mg

Sulfo-Cyanine7 maleimide, 25 mg

Sulfo-Cyanine7 maleimide, 50 mg

Sulfo-Cyanine7 maleimide, 100 mg

Sulfo-Cyanine7 NHS ester, 1 mg

Sulfo-Cyanine7 NHS ester, 5 mg

Sulfo-Cyanine7 NHS ester, 25 mg

Sulfo-Cyanine7 NHS ester, 50 mg

Sulfo-Cyanine7 NHS ester, 100 mg

Sulfo-Cyanine7.5 alkyne, 1 mg

Sulfo-Cyanine7.5 alkyne, 5 mg

Sulfo-Cyanine7.5 alkyne, 10 mg

Sulfo-Cyanine7.5 alkyne, 25 mg

Sulfo-Cyanine7.5 alkyne, 50 mg

Sulfo-Cyanine7.5 alkyne, 100 mg

Sulfo-Cyanine7.5 azide, 1 mg

Sulfo-Cyanine7.5 azide, 5 mg

Sulfo-Cyanine7.5 azide, 10 mg

Sulfo-Cyanine7.5 azide, 25 mg

Sulfo-Cyanine7.5 azide, 50 mg

Sulfo-Cyanine7.5 azide, 100 mg

Sulfo-Cyanine7.5 carboxylic acid, 1 mg

Sulfo-Cyanine7.5 carboxylic acid, 5 mg

Sulfo-Cyanine7.5 carboxylic acid, 25 mg

Sulfo-Cyanine7.5 carboxylic acid, 50 mg

Sulfo-Cyanine7.5 carboxylic acid, 100 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 100 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 1 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 25 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 50 mg

Sulfo-Cyanine7.5 dicarboxylic acid, 5 mg

Sulfo-Cyanine7.5 NHS ester, 1 mg

Sulfo-Cyanine7.5 NHS ester, 5 mg

Sulfo-Cyanine7.5 NHS ester, 25 mg

Sulfo-Cyanine7.5 NHS ester, 50 mg

Sulfo-Cyanine7.5 NHS ester, 100 mg

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資料下載

花菁類染料（Cyanine dyes）的分類介紹及選用指南

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