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DSP（57757-57-0）功能化交聯(lián)劑

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    研究人員經(jīng)常需要將蛋白質(zhì)固定在惰性表面上，用于不同的應(yīng)用，包括分離和純化ARGET大分子，選擇性去除污染物，以及酶催化和化學(xué)修飾。固定化酶在金電極上廣泛應(yīng)用于用于檢測和分析大分子的親和生物傳感器。

Chemical cross-linking is perhaps the most commonly used method for immobilizing proteins on inert surfaces. Following is a protocol for attaching a protein onto a gold surface using the chemical cross-linker dithiobis(succinimidyl propionate) . DSP is a homobifunctional, amine-reactive cross-linker. The linkage formed between DSP and the gold surface is very stable, exceeding the strength and stability of covalent silane bonds with glass. The disulfide linkage in DSP chemisorbs rapidly to gold surfaces, while the active NHS groups on either end of DSP are reactive toward primary amine groups in proteins.

化學(xué)交聯(lián)也許是zui常用的固定蛋白質(zhì)在惰性表面上的方法。以下是使用化學(xué)交聯(lián)劑DSP二硫代氨基甲酸酯（琥珀酰亞胺丙酸酯）將蛋白質(zhì)附著在金表面上的過程。DSP是一種同官能、胺反應(yīng)的交聯(lián)劑。在DSP與金表面之間形成的連接是非常穩(wěn)定的，超過了與玻璃共價硅烷鍵的強度和穩(wěn)定性。DSP中的二硫鍵化學(xué)反應(yīng)迅速地到達(dá)金表面，而DSP兩端的活性NHS基團對蛋白質(zhì)中的伯胺基團反應(yīng)。

 

產(chǎn)品名稱：DSP

簡介：Dithiobis succinimidyl propionate (DSP) is a homobifunctional crosslinker that is cell membrane permeable. DSP has amine-reactive N-hydroxysuccimide (NHS) esters at both ends of a cleava-ble, 8-atom (12.0 angstrom) spacer arm. DSP contains a reducible disulfide bond in the spacer arm that make it a good choice for various applications.

結(jié)構(gòu)式：

Molecular Weight: 404.4

CAS #: 57757-57-0

Spacer Length: 12.0 Å

Alternative Names: 3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester);

 

DSP交聯(lián)劑將蛋白質(zhì)附著在金表面上

所需材料

• DSP, 4 mg required per ml of DMSO coating solution

• Dimethylsulfoxide (DMSO), sufficient volume to cover and later rinse gold surface

• Protein solution: 1-2 mg/ml protein dissolved in a compatible buffer (pH 7-8, containing at least 50 mM NaCl).

Recommended buffers include PBS, MOPS and HEPES. Avoid buffers containing primary amines,such as Tris and glycine.

推薦的緩沖區(qū)包括PBS、MOP和HEPES。避免含有伯胺的緩沖液，如TrIS和甘氨酸。

Note: This procedure can be used to couple other ligands besides proteins that contain primary amines. For molecules that are not soluble in aqueous buffers, coupling can be performed in acetone, isopropyl alcohol or other solvents like DMSO, as long as amines are maintained in an uncharged state by addition of triethylamine or triethanolamine at a concentration equal to or greater than the concentration of DSP.

 

Procedure

1. Weigh DSP into a tube, and dissolve it in DMSO. Use 4 mg DSP per ml of DMSO necessary to cover the gold surface.

2. Incubate gold foil in dissolved DSP for 30 minutes at room temperature. 3. Rinse gold foil with DMSO and then with water. Gold foil is now activated with NHS groups. Note: Proceed directly to the next step; if protein coupling is not performed immediay, the NHS reactive groups will hydrolyze and protein coupling will be negligible.

3. Immediay add the protein solution to the activated gold foil and incubate for 1-4 hours at room temperature. Note: Incubation for longer time will not adversely affect conjugation efficiency. However, conjugation does not advance significantly after the first 1-2 hours.

4.Rinse the surface with buffer to remove cross-linker by-products (NHS leaving groups) and unconjugated protein.

Note: Free NHS groups (by-products of conjugation and hydrolysis) absorb strongly at 280 nm. Therefore, take care if attempting to determine the conjugation efficiency by measuring the change in protein concentration in solution.

應(yīng)用：

•細(xì)胞裂解前的化學(xué)交聯(lián)和免疫沉淀

• “固定”蛋白質(zhì)相互作用，以確定弱或短暫的蛋白質(zhì)相互作用

• 固定組織進行免疫染色而不損傷RNA

• 蛋白質(zhì)交聯(lián)通過單級反應(yīng)產(chǎn)生生物偶聯(lián)物

• 將蛋白質(zhì)固定在胺涂層表面上

 

產(chǎn)品列表：

（一）：Mass Spectrometry Standards - Mass Spec Standards （質(zhì)樸分析相關(guān)試劑）

Leu-Enkephalin

Gonadoliberin

Angiotensin I

Neurotensin

Peptide Calibration Standard Set

（二）：protein crosslinkers （蛋白交聯(lián)相關(guān)試劑）

Protein crosslinkers （蛋白交聯(lián)相關(guān)試劑）：

Heterobifunctional Crosslinkers異型雙功能交聯(lián)劑：

SMCC Crosslinker           CAS Number: 64987-85-5

Sulfo-SMCC Crosslinker     CAS Number: 92921-24-9

Sulfo-SANPAH Crosslinker   CAS Number: 102568-43-4

SMPH Crosslinker

PDPH Crosslinker

MBS Crosslinker            CAS Number: 58626-38-3

Sulfo-MBS Crosslinker

SPDP Crosslinker           CAS Number: 68181-17-9

LC-SPDP Crosslinker  

Sulfo-LC-SPDP Crosslinker

SIA Crosslinker            CAS Number: 39028-27-8

SBA Crosslinker            CAS Number: 42014-51-7

SMPB Crosslinker           CAS Number: 79886-55-8

BMPS Crosslinker           CAS Number: 55750-62-4

EMCS Crosslinker           CAS Number: 55750-63-5

GMBS Crosslinker           CAS Number: 80307-12-6

ANB-NOS Crosslinker        CAS Number: 60117-35-3

Homobifunctional Crosslinkers同型雙功能交聯(lián)劑：

BS3 Crosslinker           CAS Number: 82436-77-9

DSS Crosslinker           CAS Number: 68528-80-3

BS2G Crosslinker

DSG Crosslinker          CAS Number: 79642-50-5

DSP Crosslinker          CAS Number: 57757-57-0

DTSSP Crosslinker        CAS Number: 81069-02-5

Sulfo-EGS Crosslinker    CAS Number: 167410-92-6

EGS Crosslinker          CAS Number: 70539-42-3

DST Crosslinker          CAS Number: 62069-75-4

Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS：

EDC-HCl                  CAS Number: 25952-53-8

Sulfo-NHS                CAS Number: 106627-54-7

Deuterated Crosslinker BS3-d4

Deuterated Crosslinker BS2G-d4

Deuterated Crosslinker DSS-d4

（三）：Protein modification regents （蛋白修飾相關(guān)試劑）

Protein Modification Reagents 蛋白質(zhì)修飾劑：

SATA Protein Modifier

N-Succinimidyl-S-Acetyl-Thioacetate

CAS Number: 76931-93-6

Sulfo-NHS-Acetate

CAS Number: 152305-87-8

Traut's Reagent  

CAS Number: 4781-83-3

HPG

CAS Number: 24645-80-5

Iodoacetic Acid

CAS Number: 64-69-7

BMPA Modifier

CAS Number: 7423-55-4

（四）：生物素交聯(lián)試劑

Biotinylation Reagents生物素化試劑

Sulfo-NHS-Biotin     CAS Number: 119616-38-5

NHS-Biotin           CAS Number: 35013-72-0

Sulfo-NHS-LC-Biotin  CAS Number: 127062-22-0

NHS-LC-Biotin        CAS Number: 72040-63-2

Sulfo-NHS-SS-Biotin  CAS Number: 325143-98-4

NHS-SS-Biotin        CAS Number: 142439-92-7

Biotin Hydrazide     CAS Number: 66640-86-6

Biotin-HPDP          CAS Number: 129179-83-5

Iodoacetyl-LC-Biotin CAS Number: 93285-75-7

（五）Immobilization Resins固定化樹脂

Immobilization Resins固定化樹脂

Amino-Reactive Resin

Sulfhydryl-Reactive Resin

Carbohydrate-Reactive Resin

Carboxyl-Reactive Resin

Immobilized Pepsin  

Immobilized Chymotrypsin

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