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waters SEC蛋白質(zhì)分析柱

體積排阻色譜

體積排阻（又稱凝膠過(guò)濾）色譜基于蛋白質(zhì)在溶液中的體積（流體動(dòng)力學(xué)半徑）而不是分子量對(duì)其進(jìn)行分離，體積較大的物質(zhì)會(huì)在體積較小的蛋白質(zhì)之前洗脫。這種等度洗脫技術(shù)的主要機(jī)制基于填充柱中SEC顆粒的孔徑和體積。隨著目標(biāo)蛋白質(zhì)的分子量增大，應(yīng)選用顆粒和孔徑相對(duì)更大的waters SEC蛋白質(zhì)分析柱。

與其它生物分離一樣，在這類要求極為嚴(yán)苛的應(yīng)用中，相較于HPLC色譜柱，使用粒徑更小的UPLC/UHPLC色譜柱可以在更短的時(shí)間內(nèi)實(shí)現(xiàn)優(yōu)異的分離度。

BEH顆粒技術(shù)

HPLC SEC色譜柱基于沃特世亞乙基橋雜化(BEH)顆粒技術(shù)和穩(wěn)定的二醇基鍵合技術(shù)，相較于傳統(tǒng)的*硅膠基質(zhì)SEC產(chǎn)品，具有更卓。越的性能。

可對(duì)10～1500 kDa的蛋白質(zhì)進(jìn)行基于HPLC的SEC分離，且分析通量更高
出色的SEC色譜柱壽命，降低每次樣品分析的成本
出廠前經(jīng)過(guò)全面測(cè)試，可確保的色譜柱*性，使得經(jīng)過(guò)驗(yàn)證的方法更加可靠
基于ACQUITY UPLC的SEC色譜柱產(chǎn)品，可根據(jù)應(yīng)用需求實(shí)現(xiàn)無(wú)縫方法轉(zhuǎn)換

Xbridge Protein BEH SEC蛋白分析柱

出色的蛋白質(zhì)SEC分離性能

蛋白類生物治療藥物的發(fā)現(xiàn)、研發(fā)和質(zhì)量評(píng)估通常需要使用可靠、高分離度的SEC方法。200 ?和450 ?的3.5 µm Xbridge Protein BEH SEC蛋白分析柱是對(duì)分子量約10～1500 kDa的蛋白質(zhì)進(jìn)行基于HPLC的SEC分離的理想之選。

200 ?和450 ?的XBridge Protein BEH SEC蛋白分析柱裝填二醇基鍵合的3.5 µm BEH SEC顆粒，流速和壓力耐受性優(yōu)于填充5 µm以上顆粒的硅膠基質(zhì)SEC色譜柱，因此樣品通量更高。

每個(gè)生產(chǎn)批次的200 ?和450 ? 3.5 μm Xbridge Protein BEH SEC蛋白分析柱均采用相關(guān)蛋白質(zhì)標(biāo)準(zhǔn)品進(jìn)行了全面檢測(cè)，以確保*的批次間*性，保證經(jīng)過(guò)驗(yàn)證的方法采集到的數(shù)據(jù)高度可信。

ACQUITY UPLC BEH SEC色譜柱

UPLC SEC色譜柱分離

ACQUITY UPLC BEH SEC色譜柱經(jīng)過(guò)專門設(shè)計(jì)，并采用BEH蛋白質(zhì)標(biāo)準(zhǔn)品進(jìn)行了QC測(cè)試，可確保出色的批次間*性，提高經(jīng)過(guò)驗(yàn)證的方法的可信度。SEC系列產(chǎn)品是準(zhǔn)確測(cè)定治療性單克隆抗體中低濃度蛋白質(zhì)聚集體的理想之選，而且分析速度可達(dá)傳統(tǒng)HPLC SEC方法的十倍。相比之下（數(shù)據(jù)未顯示），BEH C4, 300? UPLC色譜柱則能夠分離分子量、疏水性和等電點(diǎn)各異的多種蛋白質(zhì)，同時(shí)zui大限度提高回收率并減少蛋白質(zhì)殘留。

BioSuite超高分離度(UHR)色譜柱

體積排阻色譜柱（硅膠基質(zhì)）

BioSuite SEC填料的分子量排阻限值主要取決于色譜柱中硅膠基質(zhì)材料的孔徑。此外，SEC填料的粒徑和色譜柱柱長(zhǎng)也是決定分離效率的重要參數(shù)。在分離效率方面，BioSuite UHR色譜柱（校準(zhǔn)表中未顯示）*，其次是BioSuite HR和標(biāo)準(zhǔn)SEC色譜柱。

Protein-Pak SEC色譜柱

Protein-Pak SEC色譜柱中填充硅膠基質(zhì)、二醇基鍵合的10 µm顆粒，提供60 ?、125 ?和300 ?三種孔徑，非常適用于分析級(jí)或?qū)嶒?yàn)室規(guī)模的生物大分子純化。

訂貨信息

貨號(hào)	描述
186002164	BioSuite SEC Column, 125?, 5 µm, 7.8 mm X 300 mm, 5K - 150K, 1/pkg
186002167	BioSuite SEC Guard Column, 5 µm, 6 mm X 40 mm, 1/pkg
186002166	BioSuite High Resolution SEC Column, 450?, 8 µm, 7.8 mm X 300 mm, 20K - 7M, 1/pkg
186006506	ACQUITY UPLC Protein BEH SEC Column, 125?, 1.7 µm, 4.6 mm X 300 mm, 1K - 80K, 1/pkg
186002161	BioSuite SEC Column, 125?, 4 µm, 4.6 mm X 300 mm, 5K - 150K, 1/pkg
186006505	ACQUITY UPLC Protein BEH SEC Column, 125?, 1.7 µm, 4.6 mm X 150 mm, 1K - 80K, 1/pkg
186006504	ACQUITY UPLC Protein BEH SEC Guard Column, 125?, 1.7 µm, 4.6 mm X 30 mm, 1K - 80K, 1/pkg
186002163	BioSuite SEC Guard Column, 4 µm, 4.6 mm X 35 mm, 1/pkg
186002162	BioSuite Ultra High Resolution SEC Column, 250?, 4 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pkg
186005793	ACQUITY UPLC Protein BEH SEC Guard Column, 200?, 1.7 µm, 4.6 mm X 30 mm, 10K - 500K, 1/pkg
186006851	ACQUITY UPLC Protein BEH SEC Column, 450?, 2.5 µm, 4.6 mm X 150 mm, 100K - 1500K, 1/pkg
186006850	ACQUITY UPLC Protein BEH SEC Guard Column, 450?, 2.5 µm, 4.6 mm X 30 mm, 100K - 1500K, 1/pkg
186006852	ACQUITY UPLC Protein BEH SEC Column, 450?, 2.5 µm, 4.6 mm X 300 mm, 100K - 1500K, 1/pkg
186008471	ACQUITY UPLC Protein BEH SEC Column, 200?, 1.7 µm, 2.1 mm X 150 mm, 1/pkg
186002168	BioSuite SEC Column, 125?, 10 µm, 7.5mm X 300 mm, 5K - 150K, 1/pkg
186002169	BioSuite SEC Column, 125?, 13 µm, 21.5 mm X 300 mm, 1/pkg
186005225	ACQUITY UPLC Protein BEH SEC Column, 200?, 1.7 µm, 4.6 mm X 150 mm, 10K - 500K, 1/pkg
186005226	ACQUITY UPLC Protein BEH SEC Column, 200?, 1.7 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pkg
186002170	BioSuite SEC Column, 250?, 10 µm, 7.5mm X 300 mm, 10K - 500K, 1/pkg
186002171	BioSuite SEC Column, 250?, 13 µm, 21.5 mm X 300 mm, 10K - 500K, 1/pkg
186002172	BioSuite SEC Column, 450?, 13 µm, 7.5mm X 300 mm, 20k - 1M, 1/pkg
186002173	BioSuite SEC Column, 450?, 17 µm, 21.5 mm X 300 mm, 20K - 7M, 1/pkg
186002174	BioSuite SEC Guard Column, 10 µm, 7.5mm X 75 mm, 1/pkg
186002175	BioSuite SEC Guard Column, 13 µm, 21.5 mm X 75 mm, 1/pkg
WAT011787	Protein-Pak 300SW Glass Column, 10 µm, 8 x 300 mm
WAT085250	Protein-Pak SEC Column, 60?, 10 µm, 7.8 mm X 300 mm, 500 - 20K, 1/pkg
176003596	XBridge Protein BEH SEC Column, 200?, 3.5 µm, 7.8 mm X 300 mm, and BEH 200? Protein Standard Mix
176003595	XBridge Protein BEH SEC Column, 200?, 3.5 µm, 7.8 mm X 150 mm, and BEH 200? Protein Standard Mix
WAT034279	Shodex KS-804 Column, 7 µm, 8 x 300 mm
WAT080013	Protein-Pak SEC Column, 300?, 10 µm, 7.5mm X 300 mm, 10K - 500K, 1/pkg
186007637	XBridge Protein BEH SEC Column, 125?, 3.5 µm, 7.8 mm X 300 mm
WAT034276	Shodex KS-801 Column, 7 µm, 8 x 300 mm
186000926	Protein-Pak SEC Sentry Guard Cartridge, 125?, 10 µm, 3.9 mm X 20 mm, 2/pkg
186007635	XBridge Protein BEH SEC Guard Column, 125?, 3.5 µm, 7.8 mm X 30 mm
WAT034277	Shodex KS-802 Column, 7 µm, 8 x 300 mm
176003599	XBridge Protein BEH SEC Column, 450?, 3.5 µm, 7.8 mm X 300 mm, and BEH450 Protein Standard Mix
176003598	XBridge Protein BEH SEC Column, 450?, 3.5 µm, 7.8 mm X 150 mm, and BEH450 Protein Standard Mix
WAT025830	Protein-Pak 60A SEC Column, 10 µm, 19x300 mm, 1/pkg
WAT011786	Protein-Pak 200SW SEC Glass Column, 10 µm, 8x300 mm, 1/pkg
WAT025831	Protein-Pak 125 Column, 10 µm, 19 x 300 mm
WAT035943	Shodex KW-802.5 Column, 5 µm, 8 x 300 mm
WAT035946	Shodex KW-803 Column, 5 µm, 8 x 300 mm