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waters 肽分析離子交換色譜柱

離子交換色譜

離子交換色譜基于電荷差異進行肽分離，與傳統(tǒng)的反相色譜相比，更適合需要不同/正交分離機制的分析。

通常情況下，采用鹽濃度不斷增加的梯度進行分離，體積較小且所帶電荷較少的肽會在較長的肽片段之前洗脫。與反相肽分離不同，洗脫液的pH會顯著影響分離，因此需要進行方法開發(fā)以獲得滿意的色譜結(jié)果。

waters 肽分析離子交換色譜柱與其它生物分離一樣，相比基于HPLC的色譜柱，使用粒徑更小的UPLC/UHPLC色譜柱可以在更短的時間內(nèi)實現(xiàn)優(yōu)異的組分分離度。

Protein-Pak HR離子交換玻璃色譜柱

Protein-Pak HR填料基于具有1000 ?大孔徑的剛性、親水性聚甲基丙烯酸酯顆粒。這種天然的親水性聚合物可減少非特異性吸附，從而實現(xiàn)蛋白質(zhì)質(zhì)量和生物活性的定量回收。這些填料可兼容pH 2～12的緩沖液，而且能夠耐受腐蝕性溶液。

Protein-Pak HR離子交換劑可提供：

Q官能團，強陰離子交換劑
DEAE，弱陰離子交換劑
SP，強陽離子交換劑
CM，弱陽離子交換劑

Protein-Pak HR 8 μm和15 μm填料可預(yù)填充于沃特世高級純化(AP)玻璃色譜柱中。這些色譜柱與多種分析型及制備型LC儀器兼容。

BioSuite離子交換HPLC色譜柱

BioSuite離子交換色譜柱包括四種分離填料 - SP強陽離子交換劑和CM弱陽離子交換劑，以及Q強陰離子交換劑和DEAE弱陰離子交換劑 - 這些填料都與pH穩(wěn)定（pH 2～12）的甲基丙烯酸酯類聚合物樹脂鍵合。廣泛的分離填料選擇為色譜工作者提供了靈活性，使其能夠開發(fā)出基于微小電荷差異分離蛋白質(zhì)或肽的方法。部分BioSuite離子交換色譜柱還附帶PEEK硬件，此外，還提供用于實驗室級制備型應(yīng)用的21.5 mm內(nèi)徑色譜柱

訂貨信息

貨號	描述
186004930	Protein-Pak Hi Res SP, 7µm, 4.6x100 mm, 1/pkg
WAT010640	Protein-Pak DEAE 5PW Column, 12.5 µm, 21.5x150 mm, 1/pkg
WAT038564	Protein-Pak DEAE 15HR Glass Column, 15 µm, 10x100 mm, 1/pkg
186002180	BioSuite DEAE Anion-exchange Column 1000? 10 µm, 7.5mm x 75 mm, 1/pkg
WAT039791	Protein-Pak DEAE 8HR Glass Column, 8 µm, 5x50 mm, 1/pkg
186002181	BioSuite DEAE Anion-exchange Column, 1000?, 13 µm, 21.5 mm X 150 mm, 1/pkg
WAT039790	Protein-Pak CM 8HR Glass Column, 8 µm, 5x50 mm, 1/pkg
186002184	BioSuite SP Cation-exchange Column, 1000?, 10 µm, 7.5mm X 75 mm, 1/pkg
186002185	BioSuite SP Cation-exchange Column, 1000?, 13 µm, 21.5 mm X 150 mm, 1/pkg
186002182	BioSuite Cation-exchange Column, 1300?, 7 µm, 4.6 mm X 50 mm, 1/pkg
186002183	BioSuite Cation-exchange Column, Non-porous, 2.5 µm, 4.6 mm X 35 mm, 1/pkg
186002186	BioSuite CM Cation-exchange Column, 1000?, 10 µm, 7.5mm X 75 mm, 1/pkg
186002187	BioSuite CM Cation-exchange Column, 1000?, 13 µm, 21.5 mm X 150 mm, 1/pkg
WAT011783	Protein-Pak DEAE Glass Column, 10 µm, 8x75 mm, 1/pkg
186002179	BioSuite Anion-exchange Column, Non-porous, 2.5 µm, 4.6 mm X 35 mm, 1/pkg
WAT035650	Protein-Pak DEAE 8HR Glass Column, 8 µm, 10x100 mm, 1/pkg
WAT039785	Protein-Pak CM 8HR Glass Column, 8 µm, 5x100 mm, 1/pkg
WAT039786	Protein-Pak DEAE 15HR Glass Column, 15 µm, 5x100 mm, 1/pkg
WAT039787	Protein-Pak CM 15HR Column, 15 µm, 5 mm x 50 mm
WAT088044	Protein-Pak DEAE 5PW Column, 10 µm, 7.5x75 mm, 1/pkg
WAT039780	Protein-Pak DEAE 15HR Glass Column, 15 µm, 5x50 mm, 1/pkg
186002176	BioSuite Anion-exchange Column, 4000?, 10 µm, 4.6 mm X 50 mm, 1/pkg
186002177	BioSuite Q Anion-exchange Column 1000? 10 µm, 7.5mm x 75 mm, 1/pkg
WAT039783	Protein-Pak DEAE 8HR Glass Column, 8 µm, 5x100 mm, 1/pkg
186002178	BioSuite Anion-exchange Column, 1000?, 13 µm, 21.5 mm X 150 mm, 1/pkg
186004930	Protein-Pak Hi Res SP, 7µm, 4.6x100 mm, 1/pkg
WAT039570	Protein-Pak SP 8HR Glass Column, 8 µm, 5x50 mm, 1/pkg
WAT039630	Protein-Pak Q 8HR Glass Column, 8 µm, 5x100 mm, 1/pkg
186004931	Protein-Pak Hi Res Q, 5µm, 4.6 100 mm, 1/pkg
WAT035980	Protein-Pak Q 8HR Glass Column, 8 µm, 10x100 mm, 1/pkg
WAT039575	Protein-Pak Q 8HR Glass Column, 8 µm, 5x50 mm, 1/pkg
WAT035655	Protein-Pak SP 8HR Glass Column, 8 µm, 10x100 mm, 1/pkg
186004929	Protein-Pak Hi Res CM, 7µm, 4.6x100 mm, 1/pkg
WAT037663	Protein-Pak Q 15HR Glass Column, 15 µm, 10 100 mm, 1/pkg
WAT038567	Protein-Pak SP 15HR Glass Column, 15 µm, 10x100 mm, 1/pkg
WAT039625	Protein-Pak SP 8HR Glass Column, 8 µm, 5x100 mm, 1/pkg
WAT088043	Protein-Pak SP 5PW Column, 10 µm, 7.5x75 mm, 1/pkg
WAT039782	Protein-Pak Q 15HR Glass Column, 15 µm, 5x50 mm, 1/pkg
WAT011784	Protein-Pak SP 5PW Glass 8 x 75 mm