二苯并環(huán)辛烯PEG羧基 ，DBCO-PEG-COOH，產(chǎn)品芃碩生物專業(yè)供應(yīng)。

（備注：非常規(guī)分子量：400、600。精確聚合度的小分子定制。）

結(jié)構(gòu)式：

基本信息：

中文名稱：二苯并環(huán)辛烯-聚乙二醇-羧基 (DBCO-PEG-COOH)

別名：DBCO-PEG-COOH

英文名稱：DBCO-PEG-COOH，DBCO-PEG-ACID

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

純度：95%以上

二苯并環(huán)辛烯PEG羧基 ，DBCO-PEG-COOH，產(chǎn)品芃碩生物專業(yè)供應(yīng)。

應(yīng)用簡(jiǎn)介：

Ponsure’DBCO (dibenzocyclooctyne) PEG acid, DBCO-PEG-COOH, is a heterobifunctional reactive PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneously without the need for toxic Cu catalysts. Carboxylic acid group, on the other hand, can be activated to react with amines, hydroxy and other functional groups.

Copper-free Click reaction procedures:

Prepare the azide-containing reagents in reaction buffer.

Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4℃.

Purify conjugates either by dialysis or size-exclusion chromatograph.

物理性質(zhì)：

狀態(tài)：淡黃色到白色固體，或者半固體，取決于分子量。

溶解性：溶于水，DMSO等溶劑。

保持條件：-20℃*保存，避光，干燥。

注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干。

反應(yīng)性：DBCO與含有N3基團(tuán)的反應(yīng)。COOH可以和NH2、OH等反應(yīng)。

二苯并環(huán)辛烯PEG羧基 ，DBCO-PEG-COOH，產(chǎn)品芃碩生物專業(yè)供應(yīng)。

技術(shù)指標(biāo)：

純度（PEG）：95%以上

取代率（DBCO）：90%以上

取代率（COOH）：95%以上

分散系數(shù)（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF

相關(guān)產(chǎn)品*：

MPEG-COOH

HOOC-PEG-COOH

4ARM-PEG-COOH

6ARM-PEG-COOH

8ARM-PEG-COOH

HO-PEG-COOH

BOC-NH-PEG-COOH

FMOC-NH-PEG-COOH

NH2-PEG-COOH

DSPE-PEG-COOH

DPPE-PEG-COOH

DMPE-PEG-COOH

DOPE-PEG-COOH

HOOC-PEG-T-butyl acetate

HS-PEG-COOH

OPSS-PEG-COOH

DA-PEG-COOH(ACRL-PEG-COOH)

ACA-PEG-COOH

ALKYNE-PEG-COOH

N3-PEG-COOH

MAL-PEG-COOH

LA-PEG-COOH

BIOTIN-PEG-COOH

SILANE-PEG-COOH

Cholesterol-PEG-COOH

FA-PEG-COOH

FITC-PEG-COOH

RB-PEG-COOH

CY5-PEG-COOH

DBCO-PEG-COOH

PLA-PEG-COOH

PCL-PEG-COOH

PLGA-PEG-COOH

Furan-PEG-COOH

Pyrene-PEG-COOH

二苯并環(huán)辛烯PEG羧基 ，DBCO-PEG-COOH，產(chǎn)品芃碩生物專業(yè)供應(yīng)。