硼酸的化學(xué)性質(zhì),質(zhì)量標(biāo)準(zhǔn)及用途

白色結(jié)晶性粉末或無色微帶珍珠狀光澤的磷片或六角三斜結(jié)晶。與皮膚接觸有滑膩感，無無氣味，味微酸苦后帶甜。露置空氣中無變化。加熱至100-105℃時(shí)失去一分子水而形成偏硼酸，于104-160℃時(shí)長(zhǎng)時(shí)間加熱轉(zhuǎn)變?yōu)榻古鹚?，更高溫度則形成無水物，300ºC時(shí)生成硼酸酐（B2O3）。溶于水、酒精、甘油、醚類及香精油中，水溶液呈弱酸性。硼酸在水中的溶解度隨溫度升高而增大，并能隨水蒸汽揮發(fā)；在無機(jī)酸中的溶解度要比在水的溶解度小。0.1mol/L水溶液pH為5.1。1g能溶于18ml冷水，4ml沸水，18ml冷醇，6ml沸醇或4ml甘油。在水中溶解度能隨鹽酸、檸檬酸和酒石酸的加入而增加。
熔點(diǎn)：169℃
沸點(diǎn)：300℃

項(xiàng)目Item                                              分析純    化學(xué)純
                                                       (AR)      (CP)
含量(H₃BO₃)Assay,%                                    ≥ 99.5    99.0
澄清度試驗(yàn)Clarity                                        合格    合格
水不溶物Insoluble matter in water                     ≤ 0.005   0.02
乙醇溶解度試驗(yàn)Solubility in ethanol                      合格    合格
甲醇不揮發(fā)物(以硫酸計(jì))Novolatile with methanol,%      ≤ 0.05    0.3
氯化物(Cl)Chloride,%                                  ≤ 0.0005  0.002
硫酸鹽(SO₄)Sulfate,%                                  ≤ 0.002   0.01
磷酸鹽(PO₄)Phosphate,%                                ≤ 0.0005  0.003
鈣(Ca)Calcium,%                                       ≤ 0.002   0.01
鐵(Fe)Iron,%                                          ≤ 0.0005  0.002
砷(As)Arsenic,%                                       ≤ 0.0001  0.0005
鉛(Pb)Lead,%                                          ≤ 0.001   0.003


項(xiàng)目                                                  優(yōu)級(jí)純  
                                                       (GR) 
澄清度試驗(yàn)Clarity                                      合格  
鈣(Ca)Calcium                                         ≤0.002%  
含量Assay                                             ≥99.8%  
甲醇不揮發(fā)物Novolatile with methanol                  ≤0.05%  
磷酸鹽Phosphate                                       ≤0.0005%  
硫酸鹽Sulfate                                         ≤0.0005%  
氯化物Chloride                                        ≤0.0003%  
鎂magnesium                                           ≤0.0005%  
砷(As)Arsenic                                         ≤0.00001%  
水不溶物Insoluble matter in water                     ≤0.005%  
鐵(Fe)Iron                                            ≤0.0001%  
乙醇溶解試驗(yàn)Solubility in ethanol                     合格  
重金屬Heavy metals                                    ≤0.0005%  


ACS級(jí)
項(xiàng)目名稱Item                                         ACS Grade
含量Assay                                             ≥99.5%
不溶物Insoluble matter                                ≤0.005%
甲醇不揮發(fā)物Novolatile with methanol                  ≤0.05%
氯化物(Cl)Chloride(Cl)                                ≤0.001%
磷酸鹽(PO₄)Phosphate                                  ≤0.001%
硫酸(SO₄)Sulfate                                      ≤0.01%
重金屬(以Pb計(jì))Heavy metals(as Pb)                     ≤0.001%
鐵(Fe)Iron(Fe)                                        ≤0.001%
鈣(Ca)Calcium(Ca)                                     ≤0.005%


品級(jí)Grade                               分子生物學(xué)級(jí)   
                                   (for molecular biology，≥99.5% (T) ) 
含量assay                               ≥99.5% (T) 
DNases,                                 none detected 
RNases,                                 none detected 
insoluble matter,                       passes filter test 
phosphatases,                           none detected 
proteases,                              none detected   
non-volatile substances in MeOH/HCl     <0.05%  
干燥損失loss on drying                  ≤0.5% , 20 °C (HV) 
pH                                      3.6-4.4 (25 °C, saturated solution in H2O) 
水溶性solubility H2O:                   soluble1 M, clear, colorless (hot) 
氯chloride (Cl-):                       ≤5 ppm 
磷酸根phosphate (PO43-):                ≤5 ppm 
硫酸根sulfate (SO42-):                  ≤5 ppm
  Al:                                   ≤5 ppm 
  As:                                   ≤0.5 ppm 
  Ba:                                   ≤5 ppm 
  Bi:                                   ≤5 ppm 
  Ca:                                   ≤10 ppm 
  Cd:                                   ≤5 ppm 
  Co:                                   ≤5 ppm 
  Cr:                                   ≤5 ppm 
  Cu:                                   ≤5 ppm 
  Fe:                                   ≤5 ppm 
  K:                                   ≤50 ppm 
  Li:                                   ≤5 ppm 
  Mg:                                   ≤5 ppm 
  Mn:                                   ≤5 ppm 
  Mo:                                   ≤5 ppm 
  Na:                                   ≤50 ppm 
  Ni:                                   ≤5 ppm 
  Pb:                                   ≤5 ppm 
  Sr:                                   ≤5 ppm 
  Zn:                                   ≤5 ppm 
λ 1 M in H2O 
UV absorption 
  λ: 260 nm A:                          ≤0.015 
  λ: 280 nm A:                          ≤0.010



品級(jí)Grade                               電泳級(jí)   
                               (for electrophoresis, ≥99.5%) 
外觀Appearance                          白色至類白色粉末
水溶解試驗(yàn)solubility in water           Colorless Clear ，1 g plus 25 ml, H2O 
甲醇不溶物Insoluble matter in MeOH      ≤0.005 %
非揮發(fā)物Nonvolatile Matter with MeOH    ≤0.05 % 
氯Chloride                              ≤0.001 % 
磷酸鹽Phosphate (PO4)                   ≤0.001 % 
硫酸鹽Sulfate (SO4)                     ≤0.01 % 
鈣Calcium (Ca)                          ≤0.005 % 
重金屬Heavy Metals (as Lead)            ≤0.001 % 
鐵Iron (Fe)                             ≤0.001 % 
含量Assay(% H3BO3)                      ≥99.5 % 
電泳實(shí)驗(yàn)electrophoresis test            pass



品級(jí)Grade                              細(xì)胞培養(yǎng)與植物細(xì)胞培養(yǎng)級(jí)   
                       (for cell culture and plant cell culture , ≥99.5%) 
外觀Appearance                          白色粉末
水溶解試驗(yàn)solubility in water           Clear Colorless，40 mg/ml, H2O 
純度Purity                              ≥99.5 % (Titration by NaOH) 
植物細(xì)胞培養(yǎng)測(cè)試Plant Cell Culture Test Pass 
細(xì)胞培養(yǎng)測(cè)試Cell Culture Test           Pass 



99.998% metals basis：


外觀Appearance                          白色粉末
X-射線衍射分析X-Ray Diffraction         Conforms to standard
純度Purity                              ≥99.998% Based On Trace Metals Analysis
ICP Assay                               Confirms Boron component 
總金屬雜質(zhì)Total metallic impurities     30ppm by ICP Atomic emission


99.99% metals basis:

外觀Appearance                          White Powder
X-射線衍射X-Ray Diffraction             Conforms to Structure
純度Purity                              ≥99.99% Based on Trace Metals Impurities
純度Purity (Titration by NAOH)           99.0 - 101.0 %
痕量金屬雜質(zhì)total metallic impurities   ≤100ppm by ICP Atomic emission

配制緩沖液及各種硼酸鹽，單倍體育種中配制各種培養(yǎng)基。