南通飛宇生物科技有限公司您的己糖激酶9001-51-8 試劑采購專家

己糖激酶9001-51-8
英文名稱：HK；Hexokinase from Saccharomyces cerevisiae；ATP:D-Hexose-6-phosphotransferase；Hexokinase from yeast
其他名稱：己糖磷酸激酶
CAS號：9001-51-8
分子量：~ 54 kDa (monomer)；~110 kDa (dimer)
活力：≥25units/mg protein (biuret)
活力定義：One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH：7.5～9.0
Protein by biuret：≥60%
Extinction Coefficient：E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Foreign activity：glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
性狀：白色粉末或鱗狀粉末。釀酒酵母提取。催化劑（Activators）：Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds；抑制劑（Inhibitors）：sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途：生化研究。為催化己糖6位上由ATP使之產(chǎn)生磷酸化反應(yīng)的酶。對α－D葡萄糖的親和性很高（Km=1×10－5M），對其他的己糖也可進(jìn)行磷酸化。此反應(yīng)的平衡向右偏移。△G°’=4.0千卡。該酶可被Mg2 、 K 所活化，但可被HS試劑阻抑。
保存：-20℃