說明書下載EKBD.rar

分類名稱 : 血液尿液化學,代謝產(chǎn)物分析 產(chǎn)品介紹 :

測定方法    比色法（340nm）定量測定酮體（乙酰乙酸和3 -羥基丁酸酯）的濃度。

產(chǎn)品說明    酮體（乙酰乙酸和3-羥基丁酸）主要由脂肪酸氧化, 在肝臟內產(chǎn)生，正常情況下尿液和血液中的酮體濃度很低。血液中酮體濃度升高可導致代謝性酸中毒，這往往與糖尿病、兒童低血糖、生長激素缺乏、酒精或水楊酸中毒及先天性代謝缺陷有關。簡單、直接、自動化的乙酰乙酸（AcAc）和3-羥基丁酸（BOH）檢測手段廣受親睞。本公司生產(chǎn)的酮體測試盒是基于3-羥基丁酸脫氫酶催化反應，在340nm處讀取的NADH吸光度的變化便與AcAc 和 BOH的濃度直接相關。

適用范圍    直接檢測血清、血漿、尿液和其它生物樣品中的酮體濃度。

產(chǎn)品特點    敏感且準確：用10mL樣品。采用96孔板檢測，線性檢測范圍0.12 - 8 mM酮體。簡便：檢測過程中只需加入一種工作試劑，并在室溫下讀取吸光度。高通量：該高通量96孔板檢測方法易于自動化，每天可處理大量樣品。

儲存條件：所有試劑均在-20°C下保存。

Description       Ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) are produced in the liver mainly from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypo-glycaemia, growth hormone deficiency, alcohol or salicylate intoxication and inborn errors of metabolism.Simple, direct and automation-ready procedures for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH) are very desirable. our EnzyChrom^TM ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations,

Applications       Direct assays of ketone body in serum, plasma, urine and other biological samples.

Key features        Sensitive and accurate. Uses 10 mL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature.High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.

其他 :

編號：EKBD-048,EKBD-100