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Anti α-Actin 抗體一抗說(shuō)明書(shū)
 
Catalog Number : RS-17410R    
Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）
Background:    All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene,   higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β- and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Anti α-Actin 抗體一抗說(shuō)明書(shū)
pecificity :   
– Anti α-Actin is a goat monoclonal antibody unconjugated                
– specific for α-Actin of human, mouse, rat and rabbit         
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype:  IgG
– mol wt: 43 kDa
Application :  
– Western blotting 1:100-500                
– Immunohistochemistry 1:100-500                 
– ELISA 1:500-1000                  
– Optimal working dilutions must  be determined by the end user.
Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃
 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.
Anti α-Actin 抗體一抗說(shuō)明書(shū)