CD68 抗體說(shuō)明書(shū)
From: Santa Cruz
Catalog Number : RS-0649R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: The highly glycosylated 75-120 kD antigen CD68 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD68 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD68 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD68 have been reported to be generated by alternative splicing. CD68 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD68 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Specificity : · anti-CD68 is a rabbit monoclonal antibody unconjugated
· specific for CD68 of hu, rat, mo, cow, pig, dog, Rb
· epitope mapping in the C-terminalse of CD68 of hu, rat, mo, cow, pig, dog, Rb
· use for western blotting, elisa, immunoprecipitation and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 34kDa
CD68 抗體說(shuō)明書(shū)
Application: ·Western blotting 1:100-500
·Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD68 抗體說(shuō)明書(shū)
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