蛋白名稱:CD274食蟹猴, 恒河猴重組蛋白，90251-C41H-B
蛋白構(gòu)建:A DNA sequence encoding the Cynomolgus / Rhesus CD274 (XP_015292694.1) (Met1-Thr239) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表達宿主:HEK293 Cells
蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:
1.Immobilized Recombinant Cynomolgus PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 90311-C02H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus PD-L1/B7-H1 Protein (Fc & AVI Tag), Biotinylated (Cat: 90251-C41H-B), the EC50 is 12-36 ng/mL(QC tested).
2.Biotinylated Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein, hFc & AVI Tag (Cat. No. 90251-C41H-B) captured on Protein A chip, can bind Recombinant Cynomolgus PD-1 Protein, His Tag (Cat. No. 90311-C08H) with an affinity constant of 1.200 μM as determined in an SPR assay (Biacore 8K) (Routinely tested).
3.Immobilized Atezolizumab derived Anti-PDL1 Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified(Cat: 90251-C41H-B) the EC50 is 1.75-5.25 ng/mL (Routinely tested).
4.Immobilized Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C41H-B) at 5 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Recombinant Cynomolgus PD-1 Protein (His Tag), HPLC-verified (Cat: 90311-C08H) , the EC50 is 187.75-563.55 ng/mL (Routinely tested).
蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method
預(yù)測N端:Phe 19
蛋白分子量:The recombinant Cynomolgus / Rhesus CD274 consisting of 474 amino acids and has a calculated molecular mass of 53.83 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 69.1 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP號:XP_015292694.1
蛋白氨基酸序列:Met1-Thr239
蛋白標簽:C-human IgG1-Fc & AVI
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