蛋白名稱(chēng):CD3E人重組蛋白，10977-H08H
蛋白構(gòu)建:A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (NP_000724.1) precursor was fused with a polyhistidine tag at the C-terminus.
表達(dá)宿主:HEK293 Cells
蛋白純度:≥ 80 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:
1.Immobilized SP34, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3e / CD3 epsilon Protein (His Tag) (Cat: 10977-H08H), the EC50 is 4-12 ng/mL.
2.Loaded anti-CD3E antibody on ProA Biosensor, can bind Recombinant Human CD3 epsilon/CD3e Protein, His Tag (Cat. No. 10977-H08H) with an affinity constant of 3.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3.Mouse Anti-Human CD3 (SP34）captured on Protein A chip can bind Recombinant Human CD3 epsilon/CD3e Protein, His Tag (Cat. No. 10977-H08H) with an affinity constant of 1.604 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method
預(yù)測(cè)N端:Asp 23
蛋白分子量:The recombinant human CD3E consists of 115 amino acids after removal of the signal peptide and has a predicted molecular mass of 13.2 kDa. As a result of glycosylation, human CD3E migrates as an approximately 22.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP號(hào):NP_000724.1
蛋白氨基酸序列:Met1-Asp126
蛋白標(biāo)簽:C-His
義翹神州是重組蛋白研發(fā)領(lǐng)-導(dǎo)者：
一、技術(shù)平臺(tái)
①五個(gè)重組表達(dá)系統(tǒng)
②從多個(gè)物種中制備的蛋白質(zhì)，以支持動(dòng)物模型和藥物開(kāi)發(fā)
③80+ 自動(dòng)生物反應(yīng)器
④2L-2000L生物反應(yīng)器，確保高通量生產(chǎn)
⑤GMP級(jí)別的生產(chǎn)實(shí)驗(yàn)室和設(shè)施
⑥毫克到克級(jí)的生產(chǎn)規(guī)模
二、嚴(yán)格的質(zhì)量控制體系
①400多種生物活性檢測(cè)和分析方法
②分析設(shè)備，確保生物活性
③N端氨基酸序列分析能力
④高穩(wěn)定性，確保實(shí)驗(yàn)的可重復(fù)性
三、高品質(zhì)重組蛋白產(chǎn)品
①85%以上由真核細(xì)胞表達(dá)，接近結(jié)構(gòu)
②經(jīng)驗(yàn)證的生物活性
③低內(nèi)毒素水平：<0.1EU/μg，采用LAL法確定
④高純度：>95%，采用十二烷基*聚丙烯酰胺凝膠電泳（SDS-PAGE）法或高效液相色譜（HPLC）法確定
⑤不含載體：溶于PBS緩沖液，不含牛血清白蛋白（BSA）
……
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