蛋白名稱:CD3E人重組蛋白，10977-H08H
蛋白構建:A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (NP_000724.1) precursor was fused with a polyhistidine tag at the C-terminus.
表達宿主:HEK293 Cells
蛋白純度:≥ 80 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:
1.Immobilized SP34, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3e / CD3 epsilon Protein (His Tag) (Cat: 10977-H08H), the EC50 is 4-12 ng/mL.
2.Loaded anti-CD3E antibody on ProA Biosensor, can bind Recombinant Human CD3 epsilon/CD3e Protein, His Tag (Cat. No. 10977-H08H) with an affinity constant of 3.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3.Mouse Anti-Human CD3 (SP34）captured on Protein A chip can bind Recombinant Human CD3 epsilon/CD3e Protein, His Tag (Cat. No. 10977-H08H) with an affinity constant of 1.604 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
蛋白內毒素:< 1.0 EU per μg of the protein as determined by the LAL method
預測N端:Asp 23
蛋白分子量:The recombinant human CD3E consists of 115 amino acids after removal of the signal peptide and has a predicted molecular mass of 13.2 kDa. As a result of glycosylation, human CD3E migrates as an approximately 22.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP號:NP_000724.1
蛋白氨基酸序列:Met1-Asp126
蛋白標簽:C-His
義翹神州是重組蛋白研發(fā)領-導者：
一、技術平臺
①五個重組表達系統(tǒng)
②從多個物種中制備的蛋白質，以支持動物模型和藥物開發(fā)
③80+ 自動生物反應器
④2L-2000L生物反應器，確保高通量生產(chǎn)
⑤GMP級別的生產(chǎn)實驗室和設施
⑥毫克到克級的生產(chǎn)規(guī)模
二、嚴格的質量控制體系
①400多種生物活性檢測和分析方法
②分析設備，確保生物活性
③N端氨基酸序列分析能力
④高穩(wěn)定性，確保實驗的可重復性
三、高品質重組蛋白產(chǎn)品
①85%以上由真核細胞表達，接近結構
②經(jīng)驗證的生物活性
③低內毒素水平：<0.1EU/μg，采用LAL法確定
④高純度：>95%，采用十二烷基*聚丙烯酰胺凝膠電泳（SDS-PAGE）法或高效液相色譜（HPLC）法確定
⑤不含載體：溶于PBS緩沖液，不含牛血清白蛋白（BSA）
……
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