重組小鼠 Epidermal Growth Factor 蛋白產(chǎn)品說明：

純度：> 95 % as determined by SDS-PAGE  
內(nèi)毒素：< 1.0 EU per μg of the protein as determined by the LAL method  
生物活性：

1.Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.
2.Human intestinal cancer organoids were cultured with RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.  
3.Human colorectum organoids were cultured with RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.  
4.Human lung cancer organoids were cultured with FGF2(Cat#10014-HNAE), FGF4(Cat#16043-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.  
5.Human lung organoids were cultured with FGF2(Cat#10014-HNAE), FGF4(Cat#16043-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.  
6.Human cholangiocarcinomas organoids were cultured with FGF2(Cat#10014-HNAE), HGF(Cat#10463-HNAS), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.  
7.Human liver cancer organoids were cultured with FGF2(Cat#10014-HNAE), HGF(Cat#10463-HNAS), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), TGFB1(Cat#10804-HNAC), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.  
8.Human kidney cancer organoids were cultured with FGF2(Cat#10014-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.
9.Human kidney organoids were cultured with FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS), HGF(Cat#10463-HNAS), FGF4(Cat#16043-HNAE). (Routinely tested). Data provided by D1 Medical Technology.  
10.Human gastric cancer organoids were cultured with EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.  
11.Human stomach organoids organoids were cultured with EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.  
12.Human breast cancer organoids were cultured with FGF7(Cat#10210-H07E), RSPO1(Cat#11083-HNAS), IGF1(Cat#10598-HNAE), EGF(Cat#50482-MNCH), NRG1 Beta 1(Cat#11609-H01H), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.  
13.Human ovarian organoids were cultured with IGF1(Cat#10598-HNAE), NRG1 Beta 1(Cat#11609-H01H), RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.  
14.Human small intestinal organoids were cultured with IL22(Cat#13059-HNAE), FGF10(Cat#10573-HNAE), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.  
蛋白構(gòu)建: A DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.  
Accession#P01132  
表達(dá)宿主：HEK293 Cells  
種屬：Mouse  
預(yù)測 N 端：Gly  
分子量：The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.  
緩沖液：Lyophilized from sterile PBS, pH 7.4.  
Please contact us for any concerns or special requirements.  
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.  
Please refer to the specific buffer information in the hardcopy of datasheet.  
運(yùn)輸方式：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.  
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.  
穩(wěn)定性 & 儲存條件：Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.    

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北京義翹神州科技股份有限公司作為國家高新-技術(shù)企業(yè)，主要從事生物試劑研發(fā)、生產(chǎn)、銷售并提供技術(shù)服務(wù)的生物科技公司，主要業(yè)務(wù)包括重組蛋白、抗體、基因和培養(yǎng)基等產(chǎn)品，以及重組蛋白、抗體的開發(fā)和生物分析檢測等服務(wù)，同時也為制藥公司或者生物技術(shù)公司提供單克隆抗體候選藥物的臨床前規(guī)模生產(chǎn)服務(wù)。義翹神州擁有多項(xiàng)核心技術(shù)以及從研發(fā)生產(chǎn)到放大生產(chǎn)的技術(shù)平臺，為重組蛋白或者抗體試劑的高通量、高效率研發(fā)生產(chǎn)提供保障，旨在為制藥企業(yè)和生命科學(xué)研究機(jī)構(gòu)提供高質(zhì)量的生物試劑產(chǎn)品和高水平的技術(shù)服務(wù)。

北京義翹神州始終秉承"全面致力于為生命科學(xué)研究人員提供高品質(zhì)的重組蛋白和抗體等科研工具試劑以及一站式技術(shù)服務(wù)"的理念，堅持自主研發(fā)，自主生產(chǎn)，依托技術(shù)平臺，打造國際生物試劑品牌。義翹神州提供"一站式"技術(shù)服務(wù)，從基因序列到1-30克純化后單克隆抗體僅需幾周的時間，是目前范圍內(nèi)抗體生產(chǎn)速度領(lǐng)-先的企業(yè)之一。我們也為制藥公司或者生物技術(shù)公司提供單克隆抗體候選藥物的臨床前規(guī)模生產(chǎn)服務(wù)。