足底熱刺痛儀主要通過Hargreaves法檢測動物縮足潛伏期PWL。

疼痛甩尾和冷熱板實驗雖是急性疼痛熱閾值的經(jīng)典測量方法這兩種實驗至今仍然被藥理學(xué)研究采用。但這兩種方法都有一些局限性沒有在痛覺過敏的行為反應(yīng)研究中得到運(yùn)用。

足底測試代表了一種的實驗方法它集合了疼痛過敏測試的優(yōu)點(diǎn)

· 實驗時受試動物無拘束可自由活動；

· 實驗數(shù)據(jù)記錄是儀器自動感應(yīng)完成的無需人為判斷和記錄；

· 通過聚焦紅外光源于動物足底按下開關(guān)等待動物縮回受測足爪儀器可自動記錄紅外光強(qiáng)度和持續(xù)時間；

· 紅外光源設(shè)置了一個特殊的過濾器能夠過濾掉可見光譜防止可見光干擾動物影響實驗結(jié)果；

· 帶自檢裝置反饋電路能夠進(jìn)行自檢能有效避免錯誤的實驗環(huán)境；

· 實驗數(shù)據(jù)會顯示在液晶屏上數(shù)據(jù)可導(dǎo)入U盤或通過USB數(shù)據(jù)線導(dǎo)入至電腦。

· 文獻(xiàn)引用量超過2000的足底熱刺激設(shè)備；

· 數(shù)據(jù)在前板上顯示可以通過USB傳送到電腦上USB儲存設(shè)備和軟件都包含在標(biāo)準(zhǔn)的配件包里

型號37370

產(chǎn)品特點(diǎn)
· 可自動或手動記錄爪縮回時間不需要視覺評分無差錯測量精確
· 觸摸屏控制所有功能和結(jié)果查看
· 配備USB接口可單獨(dú)工作也可連接電腦使用
· 帶數(shù)據(jù)統(tǒng)計軟件可將CSV文件從直接導(dǎo)出到USB
· 爪縮回潛伏期的分辨率為0.1s
· 紅外光強(qiáng)度01-99 級間可調(diào)
· 可選配紅外熱輻射校準(zhǔn)儀用于校準(zhǔn)紅外光源
· 6只大鼠或12只小鼠同時進(jìn)行實驗

主機(jī)及測試光源

主要參數(shù)

· 操作方式按鍵
· 數(shù)據(jù)讀取液晶屏顯示
· 紅外光強(qiáng)度01-99 級間可調(diào)
· 時間分辨率0.1s
· 紅外燈泡Halogen "Bellaphot", Mod. 64607 OSRAM, 8V-50W
· 數(shù)據(jù)轉(zhuǎn)移閃存
· 電源85-264 VAC, 50-60Hz
· 工作溫度15°- 30°C
· 噪音< 70dB
· 校準(zhǔn)紅外輻射計
· 規(guī)格85x40x35 cm
· 鼠籠尺寸20x20x14cm數(shù)量3個
· 凈重13.0kg

可選配Durham大鼠束縛器配合足底熱點(diǎn)儀用于大鼠下頜部三叉神經(jīng)痛測試。

刺激強(qiáng)度值（紅外熱輻射值）對照表

（單位mW/cm2）

主要配置清單

可選配紅外熱輻射測量儀

足底熱刺痛儀的部分引用文獻(xiàn)

《Science》
1.La Montanara, Paolo, et al. "Cyclin-dependent–like kinase 5 is required for pain signaling in human sensory neurons and mouse models." Science translational medicine 12.551 (2020)eaax4846.doi:10.1126/scitranslmed.aax4846 
IF 19.32
2.Feng, Jiao, et al. "A new painkiller nanomedicine to bypass the blood-brain barrier and the use of morphine." Science advances 5.2 (2019)eaau5148.doi10.1126/sciadv.aau5148 
IF 14.96
3.Hsiao, Hung-Tsung, et al. "The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome." Journal of medical science 26 (2019)1-11. doi:10.1186/s12929-019-0576-z 
IF 12.77
4.Zhou, Luming, et al. "Reversible CD8 T cell–neuron cross-talk causes aging-dependent neuronal regenerative decline." Science 376.6594 (2022)eabd5926. doi10.1126/science.abd5926
IF 63.71

《Nature》

5.Oswald, Manfred J., et al. "Cholinergic basal forebrain nucleus of Meynert regulates chronic pain-like behavior via modulation of the prelimbic cortex." Nature Communications 13.1 (2022)5014.doi: 
IF 17.69
6.Landra-Willm, Arnaud, et al. "A photoswitchable inhibitor of TREK channels controls pain in wild-type intact freely moving animals." Nature Communications 14.1 (2023)1160.doi: 
IF 17.69
7.Nees, Timo A., et al. "Role of TMEM100 in mechanically insensitive nociceptor un-silencing." Nature Communications 14.1 (2023)1899.
doi10.1038/s41467-023-36806-4
IF 17.69
8.Zhang, Qiaosheng, et al. "A prototype closed-loop brain–machine interface for the study and treatment of pain." Nature Biomedical Engineering (2021)1-13. doi10.1038/s41551-021-00736-7
IF 29.23
9.Zhang, Su-Bo, et al. "CircAnks1a in the spinal cord regulates hypersensitivity in a rodent model of neuropathic pain." Nature communications 10.1 (2019)4119.doi:1
10.IF17.69  
11.Jiang, Wenhao, et al. "PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis." Bone research 10.1 (2022)27. doi:10.1038/s41413-022-00201-4
13.36
12.Bao, Yi-Ni, et al. "The dopamine D1–D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury." Experimental & Molecular Medicine 53.2 (2021)235-249.doi:10.1038/s12276-021-00563-5
IF 12.15
13.Takeda, Ikuko, et al. "Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour." Nature communications 13.1 (2022)4100.doi10.1038/s41467-022-31773-8
IF 17.69
14.Liang, Hai-Ying et al. “nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors." Nature communications vol. 11,1 2501. 19 May. 2020, doi:10.1038/s41467-020-16198-5 doi:10.1038/s41467-020-16198-5
IF 17.69
15.Zhou, Hang, et al. "A sleep-active basalocortical pathway crucial for generation and maintenance of chronic pain." Nature Neuroscience (2023)1-12. doi10.1038/s41593-022-01250-y
IF 28.77
16.Wang, Yan et al. “TRPV1 SUMOylation regulates nociceptive signaling in models of inflammatory pain." Nature communications vol. 9,1 1529. 18 Apr. 2018, doi10.1038/s41467-018-03974-7 
IF 17.69
17.Iwasaki, Mai, et al. "An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats." Nature Communications 14.1 (2023)1066. doi:10.1038/s41467-023-36641-7
IF 17.69

《Cell》
18.Zhang, Fang-Xiong et al. “BK Potassium Channels Suppress Cavα2δ Subunit Function to Reduce Inflammatory and Neuropathic Pain." Cell reports vol. 22,8 (2018)1956-1964. doi:10.1016/j.celrep.2018.01.073 
IF 10.00
19.Gui, Xianwei et al. “Botulinum toxin type A promotes microglial M2 polarization and suppresses chronic constriction injury-induced neuropathic pain through the P2X7 receptor." Cell & science vol. 10 45. 23 Mar. 2020, doi:10.1186/s13578-020-00405-3

方法學(xué)文獻(xiàn)

K.M. Hargreaves, R. Dubner, F. Brown, C. Flores and J. Joris"A New and Sensitive Method for Measuring Thermal Nociception in Cutaneous Hy-peralgesia" Pain 3277-88, 1988

D.C. Yeomans & H.K. Proudfit"Characterization of the Foot Withdrawal Response to Noxious Radiant Heat in the Rat" Pain 5985-97, 1994