Leupeptin hemisulfate (Synonyms: 亮肽素)

Leupeptin hemisulfate 是一種可透過(guò)膜的巰基蛋白酶抑制劑，可抑制 Cathepsin B, Cathepsin H 和 Cathepsin L，并損害兩性溶酶體的融合。Leupeptin hemisulfate 也具有抗yan作用。

生物活性

Leupeptin hemisulfate is a membrane-permeable thiol protease inhibitor that inhibits Cathepsin B, Cathepsin H and Cathepsin L, and also impairs amphisome-lysosome fusion^[1]. Leupeptin hemisulfate also exhibits anti-inflammatory effect^[2]

IC₅₀ & Target：Cathepsin^[1]

體內(nèi)研究(In Vivo)

Leupeptin (0-36 mg/kg; intraperitoneal injection; for 4 hours; C57BL/6NCrl male mice) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction

分子量：475.59

Formula：C₂₀H₃₈N₆O₄.1/2H₂SO₄

CAS 號(hào)：103476-89-7

equence Shortening：Ac-LLR-CHO

中文名稱(chēng)：亮肽素

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式：

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)

H₂O : 83.33 mg/mL (175.21 mM; ultrasonic and warming and heat to 60°C)

參考文獻(xiàn)

[1]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42.

[2]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.

注：產(chǎn)品僅用于科研