M-CSF 蛋白, Mouse

M-CSF 蛋白, Mouse是一種促炎性細(xì)胞因子，與受體 CSF1R 結(jié)合，參與單核/巨噬細(xì)胞的增殖，破骨細(xì)胞的誘導(dǎo)，在受損的軟骨及骨質(zhì)疏松等疾病中起到重要作用。

描述

M-CSF Protein, Mouse is a pro-inflammatory cytokine, binds to its receptor CSF1R, and is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes.

研究背景

Macrophage Colony Stimulating Factor (M-CSF) is a pro-inflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms- as a secreted glycoprotein, a cell-surface protein and a proteoglycan^[1]. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis^[2].

生物活性

The ED₅₀ is <3 ng/mL as measured by murine M-NFS-60 cells, corresponding to a specific activity of >3.3 × 10⁵ units/mg.

種屬

Mouse

表達(dá)系統(tǒng)

E. coli

蛋白編號

P07141 (K33-P187)

基因 ID

12977

中文名

重組小鼠巨噬細(xì)胞群刺激因子

同用名

rMuM-CSF; CSF-1; MGI-IM

氨基酸序列

MKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKP

分子量

Approximately 30 kDa

性狀

Lyophilized powder.

組分

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.

內(nèi)毒素含量

<0.2 EU/μg, determined by LAL method

復(fù)溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

保存條件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage

運輸條件

Room temperature in continental US; may vary elsewhere.

參考文獻(xiàn)

[1]. Hamilton JA. Colony-stimulating factors in inflammation and autoimmunity. Nat Rev Immunol. 2008 Jul;8(7):533-44.

[2]. Rioja I, et al. Potential novel biomarkers of disease activity in rheumatoid arthritis patients: CXCL13, CCL23, transforming growth factor alpha, tumor necrosis factor receptor superfamily member 9, and macrophage colony-stimulating factor. Arthritis Rheum. 2008 Aug;58(8):2257-67.

注：產(chǎn)品僅用于科研