CAS號(hào)	78821-43-9	產(chǎn)地	國產(chǎn)
規(guī)格	5mg	級(jí)別	化工級(jí)
證書	ISO系列證書

Epibrassinolide (Synonyms: 表油菜素內(nèi)酯; 24-Epibrassinolide; B1105; BP55)

Epibrassinolide (24-Epibrassinolide) 是一種廣泛存在的植物生長激素，具有緩解植物重金屬和農(nóng)藥脅迫潛力。Epibrassinolide (24-Epibrassinolide) 在各種癌細(xì)胞中是一種潛在的凋亡誘導(dǎo)劑，而不影響非腫瘤細(xì)胞生長。

生物活性

Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants^[1]. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth^[2].

IC₅₀ & Target

Plant growth regulator, Apoptosis inducer^[1]

體外研究(In Vitro)

Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells^[1].

分子量：480.68

Formula：C₂₈H₄₈O₆

CAS 號(hào)：78821-43-9

中文名稱：表油菜素內(nèi)酯；布拉西諾內(nèi)酯；歐洲油菜素

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式：

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

In Vitro:

DMSO : 100 mg/mL (208.04 mM; Need ultrasonic)

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	2.0804 mL	10.4019 mL	20.8039 mL
5 mM	0.4161 mL	2.0804 mL	4.1608 mL
10 mM	0.2080 mL	1.0402 mL	2.0804 mL

請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。

參考文獻(xiàn)

[1]. Shahzad B, et al. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. Ecotoxicol Environ Saf. 2018 Jan;147:935-944.

[2]. Obakan P, et al. Activation of polyamine catabolic enzymes involved in diverse responses against epibrassinolide-induced apoptosis in LNCaP and DU145 prostate cancer cell lines.Amino Acids. 2014 Mar;46(3):553-64.

注：產(chǎn)品僅用于科研