PolyFast Transfection Reagent 

PolyFast 轉(zhuǎn)染試劑

MCE PolyFast Transfection Reagent 采用非脂質(zhì)體陽(yáng)離子聚合物為主要成分，可高效地對(duì) DNA、RNA 進(jìn)行轉(zhuǎn)染。

Description& Advantages

MCE PolyFast Transfection Reagent consists of cationic polymers and can introduce nucleic acids (DNA or RNA) into eukaryotic cells. In polymer-based transfection, exogenous DNA forms complexes with cationic polymers that enter host cells by endocytosis. PolyFast Transfection Reagent has high-efficiency, low-toxicity transfection of many cell types, including some hard-to-transfect cells.

Publications

• •J Nanobiotechnology. 2022 Sep 19;20(1):420.

• •Comput Struct Biotechnol J. 2022 Aug 13;20:4390-4401.

• •Aquaculture. 5 October 2022, 738900.

• •Research Square Print. 2022 May.

Storage

Store at -20°C for 2 years. Avoid repetitive freeze-thaw cycles.

Protocol

1.    Prepare cells

1.1    Inoculate cells in advance until the desnity reaches 70-90% for cell transfection.

Note: The viability and general health of cells prior to transfection significantly affect transfection result. Cells should be at least 90% viable prior to transfection and have had sufficient time to recover from passaging.

1.2    Remove the medium. Wash twice with PBS, and then add 900 μL serum-free medium to each well of 6-well plate.

2.    Prepare PolyFast Transfection Reagent/DNA complex

2.1    According to table 1, dilute 3 μL PolyFast Transfection Reagent with 50 μL serum-free medium for each well of 6-well plate and mix gently. Incubate at room temperature for 5 minutes.

2.2    According to table 1, dilute 1 μg DNA with 50 μL serum-free medium for each well of 6-well plate and mix gently. Incubate at room temperature for 5 minutes.

2.3    Mix the diluted PolyFast Transfection Reagent and DNA gently. Incubate at room temperature for 15 minutes.

3.    Add PolyFast Transfection Reagent/DNA complex to cells

Add the PolyFast Transfection Reagent/DNA complex to cells in 6-well plate and mix well. Incubate at 37°C for 24-48 hours. The medium can be replaced with fresh serum-containing medium after 6 hours if necessary.

Note: 1) Generally, the ratio of DNA (μg) to PolyFast Transfection Reagent (μL) is 1:3, and the transfection effect can be optimized in the range of 1:1 to 1:5 if necessary.

          2) RNA transfection just follows the protocol as described for DNA.

4.    Detection

Measure transfection efficiency using an appropriate assay for the reporter gene. For transient transfection, cells are typically assayed 24–48 hours after transfection.

注：產(chǎn)品僅用于科研