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更新時(shí)間:2024-06-26 16:19:44瀏覽次數(shù):308次
聯(lián)系我時(shí),請(qǐng)告知來自 制藥網(wǎng)| CAS號(hào) | 50-28-2 | 產(chǎn)地 | 國(guó)產(chǎn) |
|---|---|---|---|
| 規(guī)格 | 1g | 級(jí)別 | 化工級(jí) |
| 證書 | ISO系列證書 |
Estradiol (β-Estradiol) 是一種類固醇激素和主要的女性性激素。Estradiol 可上調(diào)人子宮內(nèi)膜干細(xì)胞 (hEnSCs) 神經(jīng)標(biāo)志物的表達(dá)并促進(jìn)其神經(jīng)分化。Estradiol 可用于癌癥、神經(jīng)退行性疾病和神經(jīng)組織工程的相關(guān)研究。

生物活性
Estradiol (β-Estradiol) is a steroid hormone and the major female sex hormone. Estradiol can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering[1][2].
IC50 & Target
Human Endogenous Metabolite
體外研究(In Vitro)
Estradiol (10 nM, 7 days) induces neural differentiation and increased neurite branching of human endometrial stem cells (hEnSCs)[1].
Estradiol (17β-estradiol, 10 nM,
Cell Differentiation Assay[1]
| Cell Line: | Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue |
| Concentration: | 10 nM |
| Incubation Time: | 7 days |
| Result: | Increased the number of neurite processes including neural differentiation and neurite branching. |
體內(nèi)研究(In Vivo)
Estradiol (1 nM, the hippocampal slices from FBN-ARO-KO mice) rescues long-term potentiation (LTP) amplitude[1].
Estradiol (0.0167 mg, implanted s.c., FBN-ARO-KO mice) rescues the molecular and functional deficits in FBN-ARO-KO mice[1].
| Animal Model: | FBN-ARO-KO Mice[2] |
| Dosage: | 1 nM |
| Administration: | Treated for the hippocampal slices |
| Result: | Rescued long-term potentiation (LTP) amplitude of both male and female mice. |
分子量:272.38
Formula:C18H24O2
CAS 號(hào):50-28-2
中文名稱:雌二醇;強(qiáng)力求偶素
運(yùn)輸條件:Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
DMSO : ≥ 50 mg/mL (183.57 mM)
Ethanol : 20 mg/mL (73.43 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.6713 mL | 18.3567 mL | 36.7134 mL |
| 5 mM | 0.7343 mL | 3.6713 mL | 7.3427 mL |
| 10 mM | 0.3671 mL | 1.8357 mL | 3.6713 mL |
請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí),請(qǐng)?jiān)?6 個(gè)月內(nèi)使用,-20°C 儲(chǔ)存時(shí),請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液,再依次添加助溶劑:
——為保證實(shí)驗(yàn)結(jié)果的可靠性,澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件,適當(dāng)保存;體內(nèi)實(shí)驗(yàn)的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶
請(qǐng)依序添加每種溶劑: corn oil
Solubility: 12.5 mg/mL (45.89 mM); Suspended solution; Need ultrasonic
請(qǐng)依序添加每種溶劑: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
請(qǐng)依序添加每種溶劑: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
請(qǐng)依序添加每種溶劑: 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
請(qǐng)依序添加每種溶劑: 5% DMSO 40% PEG300 5% Tween-80 50% saline
Solubility: 2.5 mg/mL (9.18 mM); Suspended solution; Need ultrasonic
請(qǐng)依序添加每種溶劑: 5% DMSO 95% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution
參考文獻(xiàn)
[1]. Elham Hasanzadeh. Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells. Cell Biol Int. 2021 Jan;45(1):140-153.
[2]. Yujiao Lu, et al. Neuron-Derived Estrogen Regulates Synaptic Plasticity and Memory. J Neurosci. 2019 Apr 10;39(15):2792-2809.
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