CAS號(hào)	25013-16-5	產(chǎn)地	國(guó)產(chǎn)
規(guī)格	500mg	級(jí)別	化工級(jí)
證書	ISO系列證書

Butylhydroxyanisole (Synonyms: 丁基羥基茴香醚; Butylated hydroxyanisole; BHA; E320)

Butylhydroxyanisole (Butylated hydroxyanisole) 是一種抗氧化劑，用作食品防腐劑。Butylhydroxyanisole 能介導(dǎo)肝毒性、生殖qi官發(fā)育和學(xué)習(xí)遲緩以及睡眠不足。Butylhydroxyanisole 具有神經(jīng)毒性，能導(dǎo)致大腦和神經(jīng)發(fā)育中斷。Butylhydroxyanisole 也是一個(gè)鐵死亡 (ferroptosis) 誘導(dǎo)劑。

生物活性

Butylhydroxyanisole (Butylated hydroxyanisole) is an antioxidant used as a food additive preservative. Butylhydroxyanisole mediates liver toxicity, retardation in reproductive organ development and learning, and sleep deficit. Butylhydroxyanisole exerts neurotoxic effects and leads to disruption of the brain and nerve development^[1]^[2]^[3]. Butylhydroxyanisole is a ferroptosis inducer^[4].

體外研究(In Vitro)

Butylhydroxyanisole exerts neurotoxic effects by promoting cytosolic calcium accumulation and endoplasmic reticulum stress in astrocytes^[1].

Butylhydroxyanisole (25-100 μM; 48 hours) inhibits growth and induces death in human astrocytes^[1].
Butylhydroxyanisole (100 μM; 48 hours) decreases the expression of cell-cycle-related proteins and increased the expression of the cell cycle inhibitory protein^[1].
Butylhydroxyanisole (100 μM; 48 hours) mediates apoptotic signals in NHA-SV40LT Cells^[1].
Butylhydroxyanisole also increases the cytosolic calcium level and the expression of endoplasmic reticulum stress proteins^[1].
Butylhydroxyanisole induces testicular dysfunction in mouse testis cells by dysregulating calcium homeostasis and stimulating endoplasmic reticulum stress^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	NHA-SV40LT cells
Concentration:	0 μM, 25 μM, 50 μM, 75 μM, 100 μM
Incubation Time:	48 hours
Result:	Exerted antiproliferative effects.

體內(nèi)研究(In Vivo)

Butylhydroxyanisole (200 mg/kg; i.g.; daily; for three days) induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Five-week-old C57BL/6 mice (WT and Nrf2^-/-)^[3]
Dosage:	200 mg/kg
Administration:	Oral gavage, daily, for three days
Result:	Increased Nqo1 staining in hepatocytes, predominately in the pericentral region.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase
NCT05094817	Women′s College Hospital\|University Health Network, Toronto	Delirium in Old Age\|Alzheimer Disease\|Mild Cognitive Impairment\|Neurocognitive Disorders	February 11, 2022
NCT03826784	Carmell Therapeutics Corporation	Tibial Fractures	November 2019	Phase 3
NCT04056429	Carmell Therapeutics Corporation	Tibial Fractures	July 2020	Phase 2

分子量：180.24

性狀：Solid

Formula：C₂₂H₃₂O₄

CAS 號(hào)：25013-16-5

中文名稱：丁基羥基茴香醚；叔丁基對(duì)羥基茴香醚；丁基大茴醚

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

In Vitro:

DMSO : ≥ 100 mg/mL (554.82 mM)

H₂O : 1 mg/mL (5.55 mM; ultrasonic and warming and heat to 60°C)

* "≥" means soluble, but saturation unknown.

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	5.5482 mL	27.7408 mL	55.4816 mL
5 mM	1.1096 mL	5.5482 mL	11.0963 mL
10 mM	0.5548 mL	2.7741 mL	5.5482 mL

請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： PBS
Solubility: 100 mg/mL (554.82 mM); Clear solution; Need ultrasonic

參考文獻(xiàn)

[1]. Sunwoo Park, et al. Butylated Hydroxyanisole Exerts Neurotoxic Effects by Promoting Cytosolic Calcium Accumulation and Endoplasmic Reticulum Stress in Astrocytes. J Agric Food Chem. 2019 Aug 28;67(34):9618-9629.  [Content Brief]
[2]. Jiyeon Ham, et al. Butylated Hydroxyanisole Exerts Neurotoxic Effects by Promoting Cytosolic Calcium Accumulation and Endoplasmic Reticulum Stress in Astrocytes. Sci Total Environ. 2020 Feb 1;702:134775.  [Content Brief]
[3]. Lin Luo, et al. Butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice. Toxicol Appl Pharmacol. 2015 Nov 1;288(3):339-48.  [Content Brief]
[4]. Jennifer Yinuo Cao, et al. Mechanisms of ferroptosis. Cell Mol Life Sci. 2016 Jun;73(11-12):2195-209.  [Content Brief]

注：產(chǎn)品僅用于科研