CAS號	98-92-0	產(chǎn)地	國產(chǎn)
規(guī)格	1g	級別	化工級

Nicotinamide (Synonyms: 煙酰an; Niacinamide; Nicotinic acid amide)

Nicotinamide 是wei生素 B3 或煙suan的一種形式，可抑制 SIRT2 的體外活性，其 EC₅₀ 值為 2 μM。 Nicotinamide 可抑制 90% 的黑色素瘤細(xì)胞數(shù)量，并增加細(xì)胞內(nèi) NAD₊、ATP、ROS 水平。Nicotinamide 抑制黑色素瘤小鼠的腫瘤生長并提高生存率，可用于黑色素瘤等皮膚ai相關(guān)的研究。

生物活性

Nicotinamide is a form of vitamin B3 or niacin that inhibits sirtuin 2 (SIRT2) activity in vitro, with an EC₅₀ of 2 μM. Nicotinamide inhibits up to 90% melanoma cell number and increases cellular NAD₊, ATP, ROS levels. Nicotinamide inhibts tumor growth in vivo and improves survival of melanoma-bearing mice, which can be used for the research of skin cancers such as melanoma^[1].

體外研究(In Vitro)

Nicotinamide (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells^[1].

Nicotinamide (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase^[1].
Nicotinamide (1-50 mM, 6 h ) increases NAD₊, ATP and ROS levels in A375 and SK-MEL-28 cells^[1].
Nicotinamide (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC₅₀ of 2 μM^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A375, SK-MEL-28, mouse B16-F10 cell
Concentration:	0, 1, 20, 50 mM
Incubation Time:
Result:	24 h, 48 h

體內(nèi)研究(In Vivo)

Nicotinamide (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model^[1].

Nicotinamide (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (subcutaneous injected with B16-F10 cells)^[1]
Dosage:	1000, 1500, 1800 mg/Kg.
Administration:	Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result:	Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight. Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase
NCT04910061	Seneque SA\|Dicentra Inc.\|LGD	Healthy Volunteers	August 5, 2021	Not Applicable
NCT05258474	University Hospital Schleswig-Holstein	Safety Issues\|Pharmacokinetic	December 4, 2020	Phase 1
NCT05194397	Children′s Hospital of Philadelphia\|National Institutes of Health (NIH)\|City of Hope Medical Center\|St. Jude Children′s Research Hospital\|National Cancer Institute (NCI)\|University of Pennsylvania	Acute Lymphoblastic Leukemia in Remission\|Cancer Survivors	November 18, 2022	Phase 2

分子量：122.12

性狀：Solid

Formula：C₆H₆N₂O

CAS 號：98-92-0

中文名稱：煙酰an

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲存方式：

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)

In Vitro:

DMSO : 50 mg/mL (409.43 mM; Need ultrasonic)

H₂O : ≥ 50 mg/mL (409.43 mM)

* "≥" means soluble, but saturation unknown.

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	8.1887 mL	40.9433 mL	81.8867 mL
5 mM	1.6377 mL	8.1887 mL	16.3773 mL
10 mM	0.8189 mL	4.0943 mL	8.1887 mL

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲存時，請在 6 個月內(nèi)使用，-20°C 儲存時，請在 1 個月內(nèi)使用。

In Vivo:

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：

——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請依序添加每種溶劑： PBS
Solubility: 100 mg/mL (818.87 mM); Clear solution; Need ultrasonic
2.

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
3.

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
4.

請依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution