產(chǎn)地	國(guó)產(chǎn)	規(guī)格	5mg
級(jí)別	化工級(jí)	證書	ISO系列證書

Tanshinone I (Synonyms: Tanshinone A)

Tanshinone I 是一種IIA型人重組 sPLA₂ 和兔重組 cPLA₂ 抑制劑，IC₅₀ 分別為 11 μM 和 82 μM。

生物活性

Tanshinone I is an inhibitor of type IIA human recombinant sPLA₂ (IC₅₀=11 μM) and rabbit recombinant cPLA₂ (IC₅₀=82 μM).

IC₅₀ & Target

IC50: 11 μM (sPLA₂), 82 μM (cPLA₂)^[1].

體外研究(In Vitro)

Tanshinone I inhibits PGE₂ formation from LPS-induced RAW macrophages (IC₅₀=38 μM). When Tanshinone I is added simultaneously with LPS, this compound clearly inhibits PGE2 production (IC₅₀=38 μM) at 10-100 μM. Tanshinone I also reduces PGE2 production (IC₅₀=46 μM) when added after COX-2 is fully induced. The fact that Tanshinone I inhibits PGE2 production by pre-induced COX-2 strongly suggests that this compound may directly inhibit COX-2 activity and/or affect PLA₂ activity. When Tanshinone I is incubated with two different forms of phospholipase A₂ (PLA₂), it clearly inhibits sPLA₂ (IC₅₀=11 μM) in a concentration-dependent manner. Although being less potent, Tanshinone I also inhibits cPLA₂ (IC₅₀=82 μM)^[1].

體內(nèi)研究(In Vivo)

Tanshinone I shows antiinflammatory activity in rat carrageenan-induced paw oedema and adjuvant-induced arthritis. In order to establish the anti-inflammatory activity of Tanshinone I, the classical animal models of acute and chronic inflammation [rat carrageenan (CGN)-induced paw oedema and rat adjuvant-induced arthritis (AIA)] are employed. When Tanshinone I is orally administered, it shows significant anti-inflammatory activity against CGN-induced paw oedema (47% inhibition at 160 mg/kg), while the IC₅₀ of indomethacin is 7.1 mg/kg. In AIA, Tanshinone I gives 27% inhibition of secondary inflammation at 18 day with an oral dose of 50 mg/kg/day, whereas prednisolone (5 mg/kg/day) shows potent inhibition (65%)^[1].

分子量：276.29

性狀：Solid

Formula：C₁₈H₁₂O₃

CAS 號(hào)：568-73-0

中文名稱：丹參酮 I

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

In Vitro:

DMSO : 2 mg/mL (7.24 mM; Need ultrasonic)

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	3.6194 mL	18.0969 mL	36.1939 mL
5 mM	0.7239 mL	3.6194 mL	7.2388 mL
10 mM	---	---	---

請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。

參考文獻(xiàn)

[1]. Kim SY, et al. Effects of Tanshinone I isolated from Salvia miltiorrhiza bunge on arachidonic acid metabolism and in vivo inflammatory responses. Phytother Res. 2002 Nov;16(7):616-20. [Content Brief]