AUTAC4

AUTAC4 是一種靶向線粒體的自噬靶向嵌合體 (AUTAC)。AUTAC4 下調(diào)細(xì)胞質(zhì)蛋白并通過線粒體自噬促進(jìn)靶向線粒體更新。

生物活性

AUTAC4 is a mitochondria-targeting autophagy-targeting chimera (AUTAC). AUTAC4 downregulates cytosolic proteins and promotes targeted mitochondrial turnover via mitophagy[1].

體外研究(In Vitro)

AUTAC4 induces K63-linked polyubiquitination. Accumulation of K63-linked polyubiquitin required approximately 8 h of incubation[1].

AUTAC4 suppresses not only cytochrome c release into the cytosol but also pro-caspase 3 cleavage. The intracellular ATP level after CCCP treatment is also maintained by AUTAC4, suggesting that mitophagy acceleration by AUTAC4 restored energy production by improving mitochondrial quality control. AUTAC4 rescues cells with acute mitochondrial injury by promoting mitophagy[1].

With the use of mito-Rosella, AUTAC4 (10 μM) can induce mitophagy in Detroit 532 cells ~24-72 h after treatment[1].

分子量

869.96

性狀

Solid

Formula

C43H48FN9O8S

CAS 號(hào)

2267315-04-6

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)

In Vitro:

DMSO : 170 mg/mL (195.41 mM; Need ultrasonic)

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量1 mg5 mg10 mg

1 mM1.1495 mL5.7474 mL11.4948 mL

5 mM0.2299 mL1.1495 mL2.2990 mL

10 mM0.1149 mL0.5747 mL1.1495 mL

*

請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。

儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和式選擇適當(dāng)?shù)娜芙夥桨浮Ｒ韵氯芙夥桨付颊?qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% saline

Solubility: ≥ 4.25 mg/mL (4.89 mM); Clear solution

2.

請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in saline)

Solubility: ≥ 4.25 mg/mL (4.89 mM); Clear solution

3.

請(qǐng)依序添加每種溶劑： 10% DMSO    90% corn oil

Solubility: ≥ 4.25 mg/mL (4.89 mM); Clear solution