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CAS號(hào)	4474-91-3	產(chǎn)地	進(jìn)口
級(jí)別	其他

注：本產(chǎn)品僅用于科研，不可用于臨床

生物活性

血管緊張素ⅱ(血管緊張素ⅱ)是一種血管收縮劑和腎素-血管緊張素系統(tǒng)的主要生物活性肽。血管緊張素ⅱ人類(lèi)中起著重要的作用在調(diào)節(jié)人體血壓,主要由血管緊張素ⅱ和G-protein-coupled受體之間的相互作用(GPCRs)血管緊張素ⅱ1型受體(AT1R)和血管緊張素ⅱ2型受體(AT2R)。血管緊張素ⅱ人類(lèi)刺激交感神經(jīng)的刺激,增加醛固酮生物合成和腎的行動(dòng)。人類(lèi)血管緊張素ⅱ誘導(dǎo)的血管平滑肌細(xì)胞,增加膠原蛋白I型和III在成纖維細(xì)胞合成,導(dǎo)致血管壁增厚和心肌纖維化。血管緊張素ⅱ人類(lèi)也誘發(fā)細(xì)胞凋亡。血管緊張素ⅱ誘導(dǎo)形成毛細(xì)血管內(nèi)皮細(xì)胞通過(guò)LOX-1依賴(lài)redox-sensitive途徑。.

IC₅₀ & Target



AT1 Receptor	AT2 Receptor

體外研究
(In Vitro)

大多數(shù)已知的血管緊張素ⅱ(Ang II)行動(dòng)由AT1受體介導(dǎo),at?受體有助于調(diào)節(jié)血壓和腎臟功能[1]
. Angiotensin II raises blood pressure (BP) by a number of actions, the most important ones being vasoconstriction, sympathetic nervous stimulation, increased aldosterone biosynthesis and renal actions. Other Angiotensin II actions include induction of growth, cell migration, and mitosis of vascular smooth muscle cells, increased synthesis of collagen type I and III in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. These actions are mediated by type 1 Ang II receptors (AT₁)^[2].
Angiotensin II (1 nM) induces the expression of LOX-1 and VEGF and enhances capillary formation from human coronary endothelial cells in Matrigel assay. Angiotensin II -mediated expression of LOX-1 and VEGF, capillary formation, intracellular reactive oxygen species generation, and phosphorylation of p38 as well as p44/42 mitogen-activated protein kinases, are suppressed by anti-LOX-1 antibody, nicotinamide-adenine dinucleotide phosphate oxidase inhibitor apocynin and the Ang II type 1 receptor blocker Losartan, but not by the Ang II type 2 receptor blocker PD123319^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Angiotensin II human (5 mL of 1 nM; intraperitoneal injection; 200-250 g Sprague-Dawley rats) induces a significant neutrophil recruitment that was maximal at 4 hours and had resolved by 24 hours^[4].
To distinguish the AT₁ receptor population that is critical for the pathogenesis of hypertension, osmotic minipumps are implanted s.c. into each animal to infuse Angiotensin II (1000 ng/kg/min) continuously for 4 weeks. Angiotensin II causes hypertension by activating AT₁ receptors in the kidney promoting sodium reabsorption^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1046.18

性狀

Solid

Formula

C₅₀H₇₁N₁₃O₁₂

CAS 號(hào)

4474-91-3

Sequence

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe

Sequence Shortening

DRVYIHPF

中文名稱(chēng)

血管緊張素Ⅱ

結(jié)構(gòu)分類(lèi)

Others

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性數(shù)據(jù)

In Vitro:

DMSO : 100 mg/mL (95.59 mM; Need ultrasonic)

H₂O : 50 mg/mL (47.79 mM; Need ultrasonic)

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	0.9559 mL	4.7793 mL	9.5586 mL
5 mM	0.1912 mL	0.9559 mL	1.9117 mL
10 mM	0.0956 mL	0.4779 mL	0.9559 mL

請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨?。以下溶解方案都?qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

1.

請(qǐng)依序添加每種溶劑： PBS
Solubility: 30 mg/mL (28.68 mM); Clear solution; Need ultrasonic
2.

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (2.39 mM); Clear solution
3.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (2.39 mM); Clear solution
4.

請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 0.83 mg/mL (0.79 mM); Clear solution