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注：本產(chǎn)品僅用于科研，不可用于臨床

Description
& Advantages

MCE Ultra High Sensitivity ECL Kit enables detection at femtogram range of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems. MCE Ultra High Sensitivity ECL Kit produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins.

Publications

•Cell Discov. 2022 Sep 6;8(1):84. [Abstract]
•Brain Behav Immun. 2022 Feb 10;102:53-70. [Abstract]
•Psychiat Res. 2023 May.
•Clin Immunol. 2022 Feb 1;236:108939. [Abstract]
•Biomed Pharmacother. 2023 Mar 16;161:114530. [Abstract]
•Oxid Med Cell Longev. 2022 Mar 29;2022:6098191. [Abstract]
•Respir Res. 2022 Jan 29;23(1):17. [Abstract]
•Cancer Cell Int. 2022 Oct 7;22(1):304. [Abstract]
•Int Immunopharmacol. 2022.
•J Neurochem. 2023 Feb 18. [Abstract]
•Neurosci Bull. 2020 Sep;36(9):972-984. [Abstract]
•Pharmaceuticals. 2023, 16(3), 361.
•Biomedicines. 2022, 10(11), 2760
•J Oncol. 2022 Aug 11;2022:5824617. [Abstract]
•Exp Cell Res. 2020 Aug 1;393(1):112059. [Abstract]
•Biol Trace Elem Res. 2022 Oct 13;1-15. [Abstract]

Storage

Stored at 4°C, and is stable for 1 year.

Protect from light.

Protocol

1 Take MCE Ultra High Sensitivity ECL Kit out of the refrigerator and allow it to equilibrate at room temperature for about 20 minutes.

2 Prepare the ECL working solution.

Note: Approximately 0.1 mL of ECL Reagent is required per cm2 of membrane area.

a) Mix solutions A and B in a 1:1 ratio. Use a in sufficient volume to ensure that the blot is completely wetted with the ECL working solution.

b) It is recommended to prepare the working solution just before use, although the mixed reagent solution is stable for several hours at room temperature.

3 Place the blot, protein-side up, in a clean container, and add ECL working solution.

4 Incubate the blot for 1-2 minutes at room temperature.

5 Drain the excess substrate and place the blot in a plastic membrane protector. Remove all air bubbles between the blot and the surface of the membrane protector.

6 Visualize the blot using X-ray film or a CCD-based imaging system.

Components