RN01-TRIpure Reagent（總RNA提取試劑）

適用于各種動(dòng)植物組織/細(xì)胞總RNA、DNA、蛋白的快速提取

產(chǎn)品介紹：

  TRIpure 試劑是直接從細(xì)胞或組織中提取總RNA的試劑。它在破碎和溶解細(xì)胞時(shí)能保持RNA的完整性。加入氯仿后離心，樣品分成水樣層和有機(jī)層。RNA存在于水樣層中。收集上面的的水樣層后，RNA可以通過(guò)異丙醇沉淀來(lái)還原。在除去水樣層后，樣品中的DNA和蛋白也能相繼以沉淀的方式還原。乙醇沉淀能析出中間層的DNA，在有機(jī)層中加入異丙醇能沉淀出蛋白。共純化DNA對(duì)于樣品間標(biāo)準(zhǔn)化RNA的產(chǎn)量十分有用。

無(wú)論是人、動(dòng)物、植物還是細(xì)菌組織，該方法對(duì)少量的組織(50-100mg)和細(xì)胞(5×106)以及大量的組織(≥1g)和細(xì)胞(>107)均有較好的分離效果。TRIpure 試劑操作上的簡(jiǎn)單性允許同時(shí)處理多個(gè)的樣品。所有的操作可以在一小時(shí)內(nèi)完成。TRIpure抽提的總RNA能夠避免DNA和蛋白的污染。故而能夠作RNA印跡分析、斑點(diǎn)雜交、poly(A)+選擇、體外翻譯、RNA酶保護(hù)分析和分子克隆。如果是用于PCR，當(dāng)兩條引物位于單一外顯子內(nèi)時(shí)，建議用擴(kuò)增級(jí)的DNase I來(lái)處理抽提的總RNA。

TRIpure 試劑能促進(jìn)不同種屬不同分子量大小的多種RNA的析出。例如，從大鼠肝臟抽提的RNA瓊脂糖凝膠電泳并用溴化乙啶染色，可見(jiàn)許多介于7 kb和15 kb之間不連續(xù)的高分子量條帶(mRNA和hnRNA成分)，兩條優(yōu)勢(shì)核糖體~5 kb (28S)和~2 kb(18S)，低分子量RNA介于0.1和0.3 kb之間 (tRNA, 5S)。當(dāng)抽提的RNA用TE稀釋時(shí)其A260/A280比值≥1.8。

適用范圍：

適用于各種動(dòng)植物組織/細(xì)胞總RNA、DNA、蛋白的快速抽提

艾德萊浙江省總代理-杭州昊鑫生物

艾德萊相關(guān)產(chǎn)品可咨詢昊鑫

RN01 TRIpure（TRIzol）總RNA提取試劑盒發(fā)表文章

1.     Effect and Molecular Mechanism of DHA on Improving Rats Learning and Memory Ability. Food Science, 2012, online 2012-11-19

2.     Differential expression of microRNAs in human keloids. Chinese Journal of Tissue Engineering Research, 2012, 16(50): 9370-9375.

3.     Study of Multidrug-resistance Reversing of Curcumin and Verapamil on Human Gastric Carcinoma SGC7901/ADR Cells.Vol.34,No.2,Apr.2012,pp.322-325

4.     Effect of Sanguisorba tannins on the expression of MGMT gene and protein in myelosuppressed mice. TUMOR Vol. 33, September 2013

5.     Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126

6.     Effects of Du Meridian Electroacupuncture on Growth Associated Protein- 43 Expression in Rats after Spinal Cord Injury. Chin J Rehabil Theory Pract, Jan. 2014,

7.     Mycobacterium Bovis Ornithine Carbamoyltransferase,MB1684, Induces Proinflammatory Cytokine Gene. Expression by Activating NF-kB in Macrophages. ORIGINAL RESEARCH ARTICLE. DNA AND CELL BIOLOGY. Volume 33, Number 5, 2014a Mary Ann Liebert, Inc.Pp. 1–9. DOI: 10.1089/dna.2013.2026.

8.     A Novel Method of Total RNA Extraction from Leaves of.China Biotechnology,2013,33(5):107-111

9.     The Response to High Magneto-Gravitational Environment of Different Differentiating-Stage Osteoblasts. Chinese Journal of Cell Biology 2013, 35(7):

10.  Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51

注：本產(chǎn)品僅用于科研，不可用于臨床