fluorescence detector is the most powerful one. the lasers are typically capable of emitting light of a very well defined wavelength with an intensity of 1-10 mw. the lif detection can be more sensitive than any other commercially available detector, even if the compound is not fluorescent itself. of course a suitable label should be available. prince technologies offers the zeta lif

some features of the zeta lif detector

• high sensitivity
• easy to use ce cell
• broad wavelength range

main specifications:

lasers    ar-ion at 488 nm and 514 nm, and he-cd at 325 nm

emission wavelength range   300-1000nm (filters)

sensitivity       10-10 m fluorescein-na yield

t broad wavelength range

the zeta lif detector allows using laser wavelengths from 325 to 800nm. moreover, because the laser is not integrated in the detector housing, it can be located in a convenient place for the user, and allows the heat generated to be dissipated quickly. this feature may also provide space savings on the bench. the laser can be remoy controlled by the detector, to provide maximum safety. for easy operation, the laser types are pre-programmed in the detector. the user needs only to se-lect the laser through the keyboard. the type of laser, and its wavelength are permanently displayed on the lcd. the laser power has a critical influence on the fluorescence signal. to ensure that adequate power is transmitted to the detection cell, a photodiode is placed inside the optical bench. the detected power is permanently displayed on the lcd screen.

high sensitivity

using a laser beam to excite the fluorescent molecules, combined with a number of advances in optical design, have made this detector"s performance substantially unsurpassed. this tremendous improvement is due, in part, to the use of the unique patented collinear optical arrangement. for example, as little as 10^-12 m rhodamine 123 can be detected easily with a signal-to-noise ratio above 10 (75µm id capillary flowcell, 488 nm argon-ion laser at 10 mw).

t easy to use ce cellhe cell is placed on the side of the detector. it is easily removable without any adjustment. a cover protects the user from the laser beam, which crosses the capillary window. the laser beam is shut when the cover is removed, so that the user has access to the cell in complete security (complying to safety regulation iec 825).

the laser is a critical choice and depends on the application the scientist has se-lected. for convenience, this choice of laser should be as broad as possible. the zeta lif detector is extremely versatile and allows using laser wavelengths from 325 to 800nm. moreover, because the laser is not integrated in the detector housing, it can be located in a convenient place for the user, and allows the heat generated to be dissipated quickly. this feature may also provide space savings on the bench.

the laser can be remoy controlled by the detector, to provide maximum safety. functions available are on/off, stand-by mode, and power control (when the laser provides this feature). an interface printed circuit board is provided for hecd and argon ion lasers. for easy operation, the laser types are pre-programmed in the detector. the user needs only to se-lect the laser through the keyboard. the type of laser, and its wavelength are permanently displayed on the lcd. the laser power has a critical influence on the fluorescence signal. to ensure that adequate power is transmitted to the detection cell, a photodiode is placed inside the optical bench. the detected power is permanently displayed on the lcd screen.

the optical layoutan optical fiber carries the laser beam to the optical bench. inside the bench, the laser beam is reflected by a dichroic mirror and is focused by a lens on the cell. the fluorescence signal is collected by the same lens and passes through the dichroic mirror. after a series of filters, the signal reaches the photomultiplier tube, which transforms the fluorescence signal into an electrical signal.