蛋白名稱:PDCD1大鼠重組蛋白,80448-R08H
蛋白構(gòu)建:A DNA sequence encoding the rat PDCD1 (NP_001100397.1) (Met1-Gln167) was expressed with a polyhistidine tag at the C-terminus.
表達(dá)宿主:HEK293 Cells
蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:
1. Measured by its ability to bind PD-L1 Protein, Human, Recombinant (ECD, His Tag), Biotinylated(Cat:10084-H08H-B) in a functional ELISA.
2. Immobilized PD-1 Protein, Rat, Recombinant (ECD, His Tag)(Cat:80448-R08H) at 2 μg/ml (100 μl/well) can bind PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag)(Cat:50010-M02H), the EC50 of human B7-H1 is 30-200 ng/mL.
蛋白內(nèi)毒素:< 1.0 EU per μg protein as determined by the LAL method.
預(yù)測N端:Leu 25
蛋白分子量:The recombinant rat PDCD1 consists of 154 amino acids and predicts a molecular mass of 17.7 kDa. It migrates as an approximately 34.8 kDa band in SDS-PAGE under reducing conditions.
蛋白NP號:NP_001100397.1
蛋白氨基酸序列:Met1-Gln167
蛋白標(biāo)簽:C-His
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