蛋白名稱:CD274狗重組蛋白，70110-D08H
蛋白構(gòu)建:A DNA sequence encoding the Canine CD274 (NP_001278901.1) (Met1-Arg236) was expressed with a polyhistidine tag at the C-terminus.
表達宿主:HEK293 Cells
蛋白純度:≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:

1.Immobilized Recombinant Canine PD-L1/B7-H1 Protein (ECD, His Tag) (Cat: 70110-D08H) at 2 μg/mL (100 μL/well) can bind Recombinant Canine PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 70109-D02H), the EC50 is 500-1400 ng/mL（Routinely tested）. 

2.Loaded Recombinant Canine PD-L1/B7-H1 Protein, His Tag (Cat. No. 70110-D08H) on NTA Biosensor, can bind Recombinant Canine PD-1 Protein, hFc Tag (Cat. No. 70109-D02H) with an affinity constant of 44.1 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method
預測N端:Phe 19
蛋白分子量:The recombinant Canine CD274 consisting of 229 amino acids and has a calculated molecular mass of 26.18 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 48.1 kDa and 41.7 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP號:NP_001278901.1
蛋白氨基酸序列:Met1-Arg236
蛋白標簽:C-His

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