K-INTDF 總膳食纖維檢測試劑盒 Inegrated Total Dietary Fibre Assay Kit

品牌：Megazyme

貨號：K-INTDF

中文品名：總膳食纖維檢測試劑盒

品名：Inegrated Total Dietary Fibre Assay Kit

規(guī)格：100 次檢測

應用：總膳食纖維檢測試劑盒，用于總膳食纖維特定檢測和分析。

K-INTDF 是總膳食纖維測定（包括抗性淀粉和非消化寡糖）的一個完整過程（AOAC方法2009.01和2011.25）。該方法結(jié)合了AOAC分析方法2002.02、985.29、991.43，2001.03的關(guān)鍵指標。

K-INTDF 總膳食纖維檢測試劑盒 Inegrated Total Dietary Fibre Assay Kit

特定膳食纖維組分測定如下：

1、不溶性膳食纖維（IDF），高分子量的水溶性膳食纖維（SJZFP）和低分子量可溶性膳食纖維（SDFS）測定（AOAC方法2011.25） 子起生物:

2、總膳食纖維：高分子量膳食纖維（hmwdf）和低分子量可溶性膳食纖維（SDFS）的測定（AOAC方法2009.01）

這些方法中所使用的酶純度很高；不含對β-葡聚糖、果膠和木聚糖具有活性的酶。

原理：

谷物、食品、飼料及其他材料中總膳食纖維的測定。 CODEX 一類方法(2011)

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檢測方法： 水解/去除非膳食纖維組分

反應時間：~ 累計3小時的工作量(跨2個工作日)

檢測限：樣品重量的0.5-*

應用案例：子起生物:

食品配料、食品和其它食材。

方法識別：

該檢測方法已經(jīng)被AOAC (Methods 2009.01 和 2011.25), AACC (Method 32-45.01 和 32-50.01) 和CODEX (一類方法)收錄認可。

試劑盒組成：

Bottle 1: 濃縮的胰腺α-淀粉酶(貨號：E-PANAA); 2 g, 150,000 Ceralpha Units/g.

  -20°C下穩(wěn)定超過 5年

Bottle 2: 淀粉葡萄糖苷酶 (貨號：E-AMGDF) (20 mL, 3300 Units/mL)

  4°C穩(wěn)定超過3年

Bottle 3: 純化的蛋白酶 (貨號：E-BSPRT) (10 mL, 350 酪氨酸 units/mL).

  -20°C下穩(wěn)定超過 5年 子起生物:

Bottle 4: 液相保留時間標準品 [麥芽糊精與麥芽糖(4:1比例), 約5 g]

  室溫干燥下保存穩(wěn)定超過 3年

Bottle 5: D-山梨醇 (約12 g, 干粉)

  室溫干燥下保存穩(wěn)定超過 3年

Bottle 6: D-葡萄糖加D-果糖標準溶液 (5 mL, 每個糖組分0.2 mg/mL).

  4°C下穩(wěn)定超過 2年

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優(yōu)點：

*符合食品法典委員會膳食纖維定義的方法

使用高純度、標準化的酶

所有試劑制備后可以穩(wěn)定保存超過2年

提供Mega-Calc? 軟件工具用于一站式原始數(shù)據(jù)處理

價格非常具有競爭力

參考文獻：

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Determination of total dietary fibre and available carbohydrates: A rapid integrated procedure that simulates in vivo digestion.McCleary, B. V., Sloane, N. & Draga, A. (2015). Starch/St?rke, 67(9-10), 860–883.

Measurement of dietary fibre components: the importance of enzyme purity, activity and specificity. McCleary, B. V. (2001), “Advanced Dietary Fibre Technology”, (B. V. McCleary and L. Prosky, Eds.), Blackwell Science, Oxford, U.K., pp. 89-105.

Dietary fiber and available carbohydrates. McCleary, B. V. & Rossiter, P. C. (2007). “Dietary Fiber: An International Perspective for Harmonization of Health Benefits and Energy Values”, (Dennis T. Gordon and Toshinao Goda, Eds.), AACC International, Inc., pp. 31-59.

Measuring dietary fibre. McCleary, B. V. (1999). The World of Ingredients, 50-53.

Enzyme purity and activity in fibre determinations. McCleary, B. V. (1999). Cereal Foods World, 44(8), 590-596.

Importance of enzyme purity and activity in the measurement of total dietary fibre and dietary fibre components. McCleary, B. V. (2000). Journal of AOAC International, 83(4), 997-1005.

Two issues in dietary fiber measurement. McCleary, B. V. (2001). Cereal Foods World, 46(4), 164-165.

Dietary fibre analysis. McCleary, B. V. (2003). Proceedings of the Nutrition Society, 62, 3-9.

Measurement of novel dietary fibres. McCleary, B. V. & Rossiter, P. (2004). Journal of AOAC International, 87(3), 707-717.

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An integrated procedure for the measurement of total dietary fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates. McCleary, B. V. (2007). Analytical and Bioanalytical Chemistry, 389(1), 291-308.

Development and evaluation of an integrated method for the measurement of total dietary fibre.McCleary, B. V., Mills, C. & Draga, A. (2009). Quality Assurance and Safety of Crops & Foods, 1(4), 213–224.

Determination of total dietary fiber (CODEX definition) by enzymatic-gravimetric method and liquid chromatography: collaborative study. McCleary, B. V., DeVries, J. W., Rader, J. I., Cohen, G., Prosky, L., Mugford, D. C., Champ, M. & Okuma, K. (2010). Journal of AOAC International, 93(1), 221-233.

Measurement of total dietary fiber using AOAC method 2009.01 (AACC International approved method 32-45.01): Evaluation and updates. McCleary, B. V., Sloane, N., Draga, A. & Lazewska, I. (2013). Cereal Chemistry, 90(4), 396-414.

Modification to AOAC Official Methods 2009.01 and 2011.25 to allow for minor overestimation of low molecular weight soluble dietary fiber in samples containing starch. McCleary, B. V. (2014). Journal of AOAC International, 97(3), 896-901.

Dietary fibre fractions in cereal foods measured by a new integrated AOAC method. Hollmann, J., Themeier, H., Neese, U. & Lindhauer, M. G. (2013). Food chemistry, 140(3), 586-589.

Adaptation of the AOAC 2011.25 Integrated Total Dietary Fiber Assay to determine the dietary fiber and oligosaccharide content of dry edible beans. Kleintop, A. E., Echeverria, D., Brick, L. A., Thompson, H. J. & Brick, M. A. (2013). Journal of Agricultural and Food Chemistry, 61(40), 9719-9726.

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