淋巴細(xì)胞功能相關(guān)抗原3(LFA-3CD58)試劑盒齊一生物長期經(jīng)營ELISA試劑盒(免費(fèi)代測)及抗體,分子學(xué)生物試劑盒,金標(biāo)法檢測試劑盒,比色法檢測試劑盒,細(xì)胞菌株,培養(yǎng)基,細(xì)胞因子,生化試劑,耗材等生物試劑產(chǎn)品.誠信經(jīng)營,價(jià)格實(shí)惠,服務(wù)周到,質(zhì)量保障!提供各種實(shí)驗(yàn)代測服務(wù).歡迎新老客戶!齊一生物科技(上海)有限公司,專業(yè)生產(chǎn)研發(fā)生化科研試劑全國:
淋巴細(xì)胞功能相關(guān)抗原3(LFA-3CD58)試劑盒有效期:6個(gè)月
預(yù)期應(yīng)用:ELISA法定量測定血清、血漿、細(xì)胞培養(yǎng)上清或其它相關(guān)生物液體中的含量.
說明
1.試劑盒保存:-20℃(較長時(shí)間不用時(shí));2-8℃(頻繁使用時(shí)).
2.濃洗滌液低溫保存會(huì)有鹽析出,稀釋時(shí)可在水浴中加溫助溶.
3.中、英文說明書可能會(huì)有不*之處,請(qǐng)以英文說明書為準(zhǔn).
4.剛開啟的酶聯(lián)板孔中可能會(huì)含有少許水樣物質(zhì),此為正?,F(xiàn)象,不會(huì)對(duì)實(shí)驗(yàn)結(jié)果造成任何影響.
實(shí)驗(yàn)原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、生物素化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色.顏色的深淺和樣品中的該指標(biāo)呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度(OD值),計(jì)算樣品濃度.
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