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分類名稱 : 代謝產(chǎn)物分析 產(chǎn)品介紹 :
測定方法 比色法（570nm）定量測定脂肪水解的程度。
產(chǎn)品說明 肥胖癥是一種慢性疾病，其起因是過剩的能量以脂肪的形式被貯存起來，而過度肥胖容易引發(fā)Ⅱ型糖尿病，心血管疾病以及癌癥等疾病。脂肪水解是指通過分解甘油三酸酯，使其轉(zhuǎn)變?yōu)楦视秃陀坞x脂肪酸的過程。在細胞環(huán)境下快速、準確地量化脂解作用，在科研和藥物研究中應用十分廣泛。本公司所生產(chǎn)的脂肪水解檢測試劑盒通過加入一種工作試劑，可直接對脂解過程中釋放的甘油量進行測量，在570nm條件下，產(chǎn)物的顏色強度與樣本中甘油的濃度成正比。
產(chǎn)品特點 敏感、準確：采用96孔板檢測，甘油的線性檢測范圍為：（1）比色法，0.9-100 μg/mL (10-1000 μM)；（2）熒光法，0.2-5 μg/mL。 簡便、快速：整個檢測過程只需加入一種工作試劑，并在室溫下反應20分鐘。 高通量：在570nm下進行藥物檢測，*的降低了潛在干擾，并能兼容含有酚紅的培養(yǎng)基。檢測可采用96孔板或384孔板。
產(chǎn)品用途 1. 能夠直接檢測細胞培養(yǎng)基內(nèi)的甘油含量，從而分析脂肪水解作用。2. 在藥物和藥理研究中，檢測藥品對脂肪水解的影響。
儲藏條件 -20°C保存，冰運。
DESCRIPTION Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. Our adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.
KEY FEATURES Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.9-100 μg/mL (10 to 1000 μM) glycerol for colorimetric assays and 0.2 to 5 μg/mL for fluorimetric assays. Rapid and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.
APPLICATIONS Direct Assays: adipolysis (glycerol in cell culture media). Drug Discovery/Pharmacology: effects of testing drugs on adipolysis.
STORAGE CONDITIONS The kit is shipped on ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of two months after receipt.
其他 :
編號：EAPL-048, EAPL-100
測試：48T, 100T
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